| 灰毡毛忍冬工厂化快繁生产体系的建立及再生苗遗传稳定性的分子鉴定 |
| |
| 引用本文： | 陈泽雄,胡凯,刘奕清.灰毡毛忍冬工厂化快繁生产体系的建立及再生苗遗传稳定性的分子鉴定[J].园艺学报,2013,40(12):2520-2526. |
| |
| 作者姓名： | 陈泽雄 胡凯 刘奕清 |
| |
| 作者单位： | （1 重庆文理学院林学与生命科学学院，重庆 402160；2 重庆市特色植物种苗工程技术研究中心，重庆 402160） |
| |
| 摘 要： | 以灰毡毛忍冬（Lonicera macranthoides Hand.-Mazz.）腋芽为材料，建立了一套适合工厂化
生产的离体再生技术体系。该体系包括腋芽诱导培养基MB + 1.0 mg · L-1 6-BA + 0.2 mg · L-1 NAA，继代
增殖培养基MB + 1.0 mg · L-1 6-BA + 0.5 mg · L-1 IAA 和MB + 1.5 mg · L-1 6-BA + 0.8 mg · L-1 IAA 和生根
培养基1/2MB + 2.0 mg · L-1 IBA + 0.2 mg · L-1 IAA。分别从继代12、24、36 个月的再生植株中随机抽取8
株进行SRAP 分析其遗传的变化。在检出的147 条SRAP 条带中，继代12 个月的再生植株未发现变异，
继代24 个月的再生植株中有2 株发生变异，继代36 个月的再生植株中有4 株发生变异。结果表明，建
立的腋芽再生培养体系在一定继代周期内是稳定可靠的，适合灰毡毛忍冬的规模化生产。
|
| 关 键 词： | 灰毡毛忍冬 快繁体系 遗传稳定性 分子鉴定 |
Establishment of Rapid Propagation System and Molecular Identification of Genetic Stability on Lonicera macranthoides |
| |
| CHEN Ze-Xiong,HU Kai,LIU Yi-Qing.Establishment of Rapid Propagation System and Molecular Identification of Genetic Stability on Lonicera macranthoides[J].Acta Horticulturae Sinica,2013,40(12):2520-2526. |
| |
| Authors: | CHEN Ze-Xiong HU Kai LIU Yi-Qing |
| |
| Institution: | （1Department of Forestry and Life Science，Chongqing University of Arts and Sciences，Chongqing 402160，China；
2Engineering Research Center for Special Plant Seedlings，Chongqing 402160，China） |
| |
| Abstract: | An efficient mass propagation system of Lonicera macranthoides Hand.-Mazz. was
established based on axillary bud-derived meristems. The optimal media of tissue culture involving in the
bud-induction medium（MB medium supplemented with 1.0 mg · L-1 6-BA，0.2 mg · L-1NAA），subculture
medium（MB medium supplemented with 1.0 mg · L-1 6-BA，0.5 mg · L-1 IAA or 1.5 mg · L-1 6-BA，0.8
mg · L-1 IAA）and root-induction medium（1/2MB medium supplemented with 2.0 mg · L-1 IBA and 0.2
mg · L-1 IAA）were selected. Thereafter，the genetic variation of 8 plants randomly sampled from
regeneration plants cultured for 12，24 and 36 months respectively was investigated by SRAP analysis and
there were 0，25% and 50% in variation rate found in regeneration lines respectively based on the 147
bands detected by SRAP markers. The results showed that regeneration culture system can maintain
genetic stability during certain subculture cycles，indicating that it can be readily employed for large-scale
propagation. |
| |
| Keywords: | Lonicera macranthoides Lonicera macranthoides rapid propagation system genetic stability molecular identification |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
