| 赤霉素对露地栽培月季‘卡罗拉’生长发育的影响 |
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| 引用本文： | 李茂福,杨 媛,王 华,刘佳棽,金万梅.赤霉素对露地栽培月季‘卡罗拉’生长发育的影响[J].园艺学报,2019,46(4):749-752. |
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| 作者姓名： | 李茂福 杨 媛 王 华 刘佳棽 金万梅 |
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| 作者单位： | 1北京市林业果树科学研究院，农业部华北地区园艺作物生物学与种质创制重点实验室，北京 100093；2北京市落叶果树工程技术研究中心，北京 100093 |
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| 基金项目： | 北京市农林科学院青年基金项目（QNJJ201513）；北京市农林科学院科技创新能力专项（KJCX20170203，KJCX20170104，KJCX20170601 and KJCX20180202）；国家自然科学基金青年基金项目（31600418）；市科委重大项目（D131100000113001） |
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| 摘 要： | 通过喷施不同浓度的GA_3(以喷水为对照),记录初花和谢花日期,于盛花期时调查花枝长、花柄长、株高及叶片长宽比等生长指标,研究赤霉素处理对露地栽培切花月季‘卡罗拉’生长发育的影响。结果表明,不同浓度GA_3处理对其株高、花枝长、花柄长均具有不同程度的促进作用。300 mg·L~(-1) GA_3处理,平均花枝最长,叶片长宽比值最大;300和400 mg·L~(-1) GA_3处理,平均花柄最长;500 mg·L~(-1) GA_3处理,平均株高最高。对300 mg·L~(-1) GA_3处理的植株材料进行扫描电镜观察及RT-PCR分析结果发现,RhGA2ox、RhGA20ox、RhGA_3ox及RhGID1基因表达上调,表明促进了体内GA_3的积累,诱导了生长素合成相关基因的表达,导致植物细胞伸长、细胞体积增大,因而促进植株增高、叶片变长;开花相关基因RhAP1、RhKSN和RhSOC1的表达先上升后下降;RhAP1、RhFT和RhSOC1的表达,尤其是开花素基因RhFT在21 d时的高水平表达可能造成比对照提早开花;而RhKSN基因高水平表达抑制了开花基因表达导致开花期延长。
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| 关 键 词： | 月季 赤霉素 生长发育 |
Effects of Gibberellin on Growth and Development of Rose‘Carola’ |
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| LI Maofu,YANG Yuan,WANG Hua,LIU Jiashen,JIN Wanmei,.Effects of Gibberellin on Growth and Development of Rose‘Carola’[J].Acta Horticulturae Sinica,2019,46(4):749-752. |
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| Authors: | LI Maofu YANG Yuan WANG Hua LIU Jiashen JIN Wanmei |
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| Institution: | 1. The Orthopaedic Centre, The First Affiliated Hospital of Shihezi University, Shihezi 832000, China;
2. Department of Ophthalmology, The People's Hospital of Shihezi, Shihezi 832000, China |
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| Abstract: | In this study，different concentrations of gibberellin（GA3）were applied to the branch of the rose，‘Corolla’，and the water spraying was used as the control. The initial flower date，the date of flower opening，and the date of flower withering were recorded and the length of flower branches，flower stalks，plant heights and the ratio of leaf longitudinal and horizontal were measured at full-bloom stage，in order to reveal the effects of GA3 on the growth and development of rose‘Carola’. When the treatment concentration of GA3 was 300 mg · L-1，the average length of the rose was the longest and the aspect ratio of the leaf was the largest；the treatment with the concentration of 300 and 400 mg · L-1 had the longest average length of the flower stalks；the concentration 500 mg · L-1 had the highest average plant height. Under the spraying condition of GA3 300 mg · L-1，RT-PCR results demonstrated that the application of GA3 up-regulated the expression of RhGA2ox，RhGA20ox，RhGA3ox，and RhGID1 genes，which might participate in the regulation of GAs metabolism and hormone signaling. Furthermore，the changes might indirectly induce the expression of genes related to auxin synthesis，leading to cell elongation and cell volume increase，which in turn promoted the increase of the plant height and the leaf length in rose plants. The investigation of related flowering-related genes，RhAP1，RhKSN and RhSOC1 showed that the expression pattern of RhAP1，RhFT and RhSOC1 gene expression levels were first to increase and then decrease after the application of GA3，and changed greatly，compared with the control. The high expression of the flowering gene，RhFT may be one of the causes of the earlier date for flowering due to GA3 treatment；in addition，the high expression of RhKSN may be one of the reasons for the longer period of flowering. |
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| Keywords: | Rosa hybrida gibberellin growth and development |
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