| 土霉素处理对Valencia夏橙中淀粉及相关基因表达的影响 |
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| 引用本文： | 姚廷山,周 彦,Diann Achor,周常勇.土霉素处理对Valencia夏橙中淀粉及相关基因表达的影响[J].园艺学报,2019,46(2):227-236. |
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| 作者姓名： | 姚廷山 周 彦 Diann Achor 周常勇 |
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| 作者单位： | 1西南大学柑桔研究所，重庆 400712；2西南大学植物保护学院，重庆 400716；3佛罗里达大学柑橘教育与研究中心，佛罗里达 33850 |
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| 基金项目： | 国家重点研发计划项目（2018YFD0201500）；重庆市自然科学基金项目（cstc2016jcyjA0118） |
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| 摘 要： | 黄龙病(Huanglongbing,HLB)会造成柑橘韧皮部坏死堵塞,导致光合同化物运输不畅,淀粉大量积累。在感染HLB的4年生Valencia夏橙病株上分别注入0.1和0.2 g·株~(-1)土霉素(Oxytetracycline,OTC),90 d后运用qPCR检测,病株中HLB病原菌(Candidatus Liberibacter asiaticus,Las)含量均明显降低,且0.2和0.1 g·株~(-1) OTC处理的效果相当。I2/KI显色及LM观测表明,0.2 g·株~(-1) OTC处理后植株的淀粉含量从注射前的18.58μg·mm~(-2)减少至90 d的5.24μg·mm~(-2),而0.1 g·株~(-1)的处理90 d仅降至11.88μg·mm~(-2)。高效液相色谱(HPLC)检测结果表明,注射后90 d内,试验用浓度0.2 g·株~(-1) OTC在植株体内可降解至200μg·kg~(-1)以下。基因表达结果表明,注射0.2 g·株~(-1) OTC后30和90 d,淀粉合成及分解相关基因表达量均下降,其中淀粉合成相关基因AGPase表达量下降最显著,这与OTC注射后30和90 d叶片内淀粉含量下降结果一致。
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| 关 键 词： | 柑橘 黄龙病 土霉素 淀粉合成酶 淀粉分解酶 防控 |
Effect of Oxytetracycline on the Expression of Starch and Related Genes in HLB-affected Valencia |
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| YAO Tingshan,ZHOU Yan,Diann Achor,ZHOU Changyong,.Effect of Oxytetracycline on the Expression of Starch and Related Genes in HLB-affected Valencia[J].Acta Horticulturae Sinica,2019,46(2):227-236. |
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| Authors: | YAO Tingshan ZHOU Yan Diann Achor ZHOU Changyong |
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| Institution: | 1Citrus Research Institute，Southwest University，Chongqing 400712，China；2College of Plant Protection，Southwest University，Chongqing 400716，China；3Citrus Research and Education Center，University of Florida，Lake Alfred，FL. 33850，USA |
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| Abstract: | Citrus greening or Huanglongbing（HLB）is one of the most destructive diseases of citrus and causes significant economic losses worldwide. HLB can cause phloem necrosis and blockage in citrus，resulting in poor transport of photosynthetic assimilates and large accumulation of starch. In this study，0.1 g · tree-1 and 0.2 g · tree -1 oxytetracycline（OTC）were injected into the 4 year old Valencia （Citrus sinensis）infected with HLB. The qPCR detection showed that the abundance of Las（Candidatus Liberibacter asiaticus）in leaves was reduced effectively in 90 d. There was no difference of the OTC treatment effect between 0.1 g · tree-1 and 0.2 g · tree-1 OTC. I2/KI coloration and LM observation showed that the content of starch in the treated group of 0.2 g · tree-1 decreased from 18.58 μg · mm-2 to 5.24 μg · mm-2 after injection. But 0.1 g · tree-1 treatment had no significant effect on reducing starch content. The results of high performance liquid chromatography（HPLC）showed that OTC gradually degraded in the plant over time. Expression analysis showed that AGPase，GPT2，β-amylase and GBSS expressed in leaves decreased after 0.2 g · tree-1 OTC injection. Among them，AGPase has the highest decreased expression. This is consistent with the results of starch content in leaves 30 and 90 days after OTC injection. |
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| Keywords: | citrus HLB oxytetracycline starch synthase enzyme starch splitting enzyme control |
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