| 苹果MdMYB2基因的克隆及功能鉴定 |
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| 引用本文： | 曲丰佳,安建平,姚继芳,李浩浩,李媛媛,由春香,王小非,郝玉金.苹果MdMYB2基因的克隆及功能鉴定[J].园艺学报,2017,44(12):2245-2251. |
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| 作者姓名： | 曲丰佳 安建平 姚继芳 李浩浩 李媛媛 由春香 王小非 郝玉金 |
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| 作者单位： | （山东农业大学园艺科学与工程学院，作物生物学国家重点试验室，山东泰安 271018） |
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| 摘 要： | 以‘嘎拉’苹果为材料,采用同源克隆和PCR技术分离了苹果基因MdMYB2(基因序列号:MDP0000823458)。MdMYB2的开放阅读框(ORF)长度为873 bp,编码含有290个氨基酸的蛋白,预测其蛋白质分子量为32.92 kD,等电点为4.91。系统进化树分析显示,苹果MYB2与梨MYBL2亲缘关系最近,同源性最高。组织表达分析表明,MdMYB2在苹果的各个组织均有表达,在茎和果实中表达相对较高。MdMYB2的表达明显受盐胁迫的诱导。构建了MdMYB2的表达载体,并通过农杆菌介导的遗传转化得到了转基因苹果愈伤和转基因拟南芥植株。抗性试验表明,过量表达MdMYB2明显提高了转基因苹果愈伤对盐胁迫的抗性。此外,异位表达MdMYB2也能提高拟南芥对盐胁迫的抗性,以上试验结果表明MdMYB2在植物盐胁迫响应中发挥重要作用。
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| 关 键 词： | 苹果 MdMYB2 基因克隆 盐胁迫 |
Gene Cloning and Functional Identification of an Apple MdMYB2 Gene |
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| QU Fengjia,AN Jianping,YAO Jifang,LI Haohao,LI Yuanyuan,YOU Chunxiang,WANG Xiaofei,HAO Yujin.Gene Cloning and Functional Identification of an Apple MdMYB2 Gene[J].Acta Horticulturae Sinica,2017,44(12):2245-2251. |
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| Authors: | QU Fengjia AN Jianping YAO Jifang LI Haohao LI Yuanyuan YOU Chunxiang WANG Xiaofei HAO Yujin |
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| Institution: | 1. Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China;
2. Laboratory of Hepato-Pancreato-Biliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China |
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| Abstract: | MdMYB2 gene was cloned from Malus × domestica‘Gala’with homologous cloning using RT-PCR method. Its open reading frame（ORF）contained 873 bp nucleotides，which encodes a predicted protein containing 290 amino acid residues. The predicted protein has a molecular mass of 32.92 kD and a pI value of 4.91. Phylogenetic analysis demonstrated that MdMYB2 showed the highest evolutionary relationship with PbMYBL2 from pear. Expression analysis showed that the MdMYB2 gene was constitutively expressed in different tissues，with rather higher expression levels in fruit and stem than in others. Meanwhile，its expression was noticeably induced by salt stress treatment. Finally，transgenic apple calli and Arabidopsis were obtained with Agrobacterium-mediated genetic transformation. Tolerance assays indicated that overexpression of MdMYB2 remarkably enhanced the tolerance of transgenic apple calli to high salinity，further supporting that MdMYB2 positively regulates salt tolerance in apple. In addition，ectopic expression of MdMYB2 also enhanced salt stress tolerance in Arabidopsis，indicating that MdMYB2 plays an important role in plant salt stress tolerance. |
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| Keywords: | apple MdMYB2 gene clone salt stress |
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