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玉米丝黑穗病菌基因组SSR的信息分析
引用本文:王士臻,张作刚,李新凤,高俊明,王建明.玉米丝黑穗病菌基因组SSR的信息分析[J].山西农业大学学报(自然科学版),2012,32(6).
作者姓名:王士臻  张作刚  李新凤  高俊明  王建明
作者单位:山西农业大学农学院,山西太谷,030801
基金项目:山西省科技攻关项目,山西省自然科学基金项目
摘    要:利用已经公布的玉米丝黑穗病菌基因组测序的结果,对该基因组中基序为2~6碱基的微卫星(SSR)序列进行了系统的分析。结果表明,在已经公布的23条基因组中选择前3条染色体进行分析,共有888个SSR,其总长度为3.512kb,占这3条基因组染色体碱基数的0.068%,平均6.50kb中就分布有一个长度大于15bp的SSR序列。其中,数量最多的为三碱基重复序列,共有SSR数量329个,其次为六碱基重复序列,数量达185个。五碱基重复序列的SSR数量为155个,数量最少的是四碱基重复序列,只有101个。

关 键 词:玉米丝黑穗病菌  基因组  微卫星序列  频率  分布

Information Analysis on Microsatellites in the Genome of Sporisorium reilianum
Wang Shizhen , Zhang Zuogang , Li Xinfeng , Gao Junming , Wang Jianming.Information Analysis on Microsatellites in the Genome of Sporisorium reilianum[J].Journal of Shanxi Agricultural University,2012,32(6).
Authors:Wang Shizhen  Zhang Zuogang  Li Xinfeng  Gao Junming  Wang Jianming
Abstract:A total of genome of publicly available DNA sequence in Sporisorium reilianum was researched for dinucleotide to hexanucleotide simple sequence repeat(SSR) to determine the type,size and frequency.A total of 888 SSRs were observed in the first three chromosomes.Total length of SSRs was 3.512 kb,about 0.068% of these chromosomes.The SSRs occur on average about once every 3.512 kb for dinucleotide to hexanucleotide in the first three chromosomes.The most abundant microsatellites were trinucleotide repeats with the number of 329,followed by hexanucleotide and pentanucleotide repeats,and the number of these SSRs was 185 and 155,respectively.The least abundant was tetranucleotide repeat,only 101 were found.
Keywords:Sporisorium reilianum  Genome  SSR  Frequency  Distribution
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