Bioassay-guided isolation of a novel chemoattractant for <Emphasis Type="Italic">Ralstonia solanacearum</Emphasis> in tomato root exudates |
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| Authors: | Takuya Hasegawa Atsushi Okabe Yusuke Kato Atsushi Ooshiro Hiroshi Kawaide Masahiro Natsume |
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| Institution: | 1.Department of Biological Production Science, United Graduate School of Agricultural Science,Tokyo University of Agriculture and Technology,Fuchu,Japan;2.Department of Bioregulation and Biointeraction, Graduate School of Agriculture,Tokyo University of Agriculture and Technology,Fuchu,Japan;3.Okinawa Prefectural Agricultural Research Center,Itoman,Japan;4.Division of Bioregulation and Biointeraction, Institute of Agriculture,Tokyo University of Agriculture and Technology,Fuchu,Japan |
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| Abstract: | Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum. An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH2-modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis. |
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