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NAA和IBA对非洲菊组培苗生根的影响
引用本文:戴云新,张健,李敏,李玉娟.NAA和IBA对非洲菊组培苗生根的影响[J].安徽农业科学,2009,35(19):8845-8847.
作者姓名:戴云新  张健  李敏  李玉娟
作者单位:江苏省南通市蔬菜研究所,江苏南通,226001;江苏沿江地区农业科学研究所,江苏如皋,226541
摘    要:目的]探讨IBA和NAA的不同浓度组合对非洲菊生根的影响,筛选出有利于非洲菊生根的生长激素组合,提高非洲菊组培苗的生根率和生根质量。方法]以非洲菊A。的试管苗为材料,采用1/2MS分别与不同浓度的IBA、NAA配组为生根培养基,调查不同激素水平处理下非洲菊试管苗的生根数、根长、根粗、新生叶片数和鲜重,筛选出非洲菊生根的最佳培养基。结果]在IBA浓度相同时,不同浓度NAA对试管苗的生根数、平均根长和平均新生叶片数影响较大,0.1mg/LNAA诱导的根最好;在NAA浓度相同时,不同浓度的IBA对根的形态影响较小,0.4mg/LIBA诱导的根最好。结论]初步确定了非洲菊的最佳生根培养基为:1/2MS+IBA0.4mg/L+NAA0.1mg/L。

关 键 词:非洲菊  NAA  IBA

Effects of NAA and IBA on the Rooting of Tissue Culture Seedling of Gerbera jamesonii Bolus
Institution:DAI Yun-xin et al ( Nantong Institute of Vegetable Research in Jiangsu Province, Nantong, Jiangsu 226001 )
Abstract:Objective] The study aimed to discuss the effect of different concn.combinations of IBA and NAA on rooting of Gerbera jamesonii Bolus so as to screen the growth hormone combination beneficial to rooting and improve the rooting rate and rooting quality of tissue culture seedlings of G.jamesonii Bolus.Method] With the test-tube plantlets of G.jamesonii Bolus A1 as the materials, 1/2MS was matched with the different combinations of IBA and NAA with different concn.and were used as rooting culture mediums.The number of roots, root length, root diameter, number of new leaves and fresh weight of test-tube plantlets of G.jamesonii Bolus were investigated after treatment with different concn.of hormones so as to screen the optimum medium for rooting of G.jamesonii Bolus.Result] When the concn of IBA was same, NAA with different concn, had a great effect on the number of roots, average root length and average number of new leaves of test-tube plantlets of G.jamesonii Bolus and roots induced by 0.1 mg/L NAA were the best.When the concn.of NAA was same, IBA with different concn.had less effect on root form and roots induced by 0.4 mg/L IBA were the best.Conclusion] The best rooting medium for G.jamesonii Bolus was confirmed primarily as follows: 1/2MS + IBA 0.4 mg/L + NAA 0.1 mg/L.
Keywords:NAA  IBA  Gerbera jamesonii Bolus  NAA  IBA
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