Improved solvent extraction procedure and high-performance liquid chromatography-evaporative light-scattering detector method for analysis of polar lipids from dairy materials |
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| Authors: | Le Thien Trung Miocinovic Jelena Nguyen Tuyet Mai Rombaut Roeland van Camp John Dewettinck Koen |
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| Institution: | Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Ghent University , Coupure links 653, 9000 Gent, Belgium. le.trungthien@ugent.be |
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| Abstract: | A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use. |
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