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| 4株猪链球菌2型分离株主要毒力因子(MRP、EF)的全基因序列分析 | |
| 引用本文： | 倪艳秀,段志涛,何孔旺,陆承平.4株猪链球菌2型分离株主要毒力因子(MRP、EF)的全基因序列分析[J].江苏农业学报,2007,23(3):204-207. |
| 作者姓名： | 倪艳秀 段志涛 何孔旺 陆承平 |
| 作者单位： | 1. 江苏省农业科学院兽医研究所,农业部动物畜禽疫病诊断重点开放实验室,江苏,南京,210014;南京农业大学,农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 2. 江苏省农业科学院兽医研究所,农业部动物畜禽疫病诊断重点开放实验室,江苏,南京,210014 3. 南京农业大学,农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
| 基金项目： | 国家重点基础研究发展计划(973计划) , 江苏省自然科学基金 |
| 摘 要： | 猪链球菌2型(SS2)是一种重要的人兽共患病病原菌,溶菌酶释放蛋白(MRP)和胞外因子(EF)是其重要的2种毒力因子.参照GenBank上已发表的MRP和EF基因序列,设计引物,对4株国内SS2分离株进行MRP和EF全基因序列测定并进行分析.结果表明:4株SS2国内分离株MRP基因的完整开放阅读框(ORF)都为3 771 bp、编码1 256个氨基酸,EF基因的完整ORF都为2 532 bp,编码843个氨基酸;与国外参考株D282之间的MRP核苷酸和蛋白质的同源性都为99.9%,EF核苷酸的同源性为99.9%、蛋白质的同源性为99.5%～100.0%. |
| 关 键 词： | 猪链球菌2型 MRP基因 EF基因 序列分析 猪链球菌 分离株 毒力因子 全基因序列测定 序列分析 Type MRP Gene Analyses 同源性 蛋白质 核苷酸 国外 氨基酸 编码 开放阅读框 结果 设计 GenBank 胞外因子 溶菌酶释放蛋白 |
| 文章编号： | 1000-4440（2007）03-0204-04 |
| 修稿时间： | 2006-07-28 |
Sequence Analyses of MRP Gene and EF Gene of 4 Isolates of Streptoco-ccus suis Type 2 |
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| NI Yan-xiu,DUAN Zhi-tao,HE Kong-wang,LU Cheng-ping.Sequence Analyses of MRP Gene and EF Gene of 4 Isolates of Streptoco-ccus suis Type 2[J].Jiangsu Journal of Agricultural Sciences,2007,23(3):204-207. | |
| Authors: | NI Yan-xiu DUAN Zhi-tao HE Kong-wang LU Cheng-ping |
| Institution: | 1. Key Laboratory of Animal Disease Diagnosis, Ministry of Agriculture; Institute of Veterinary Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture; Nanjing Agricultural University, Nanjing 210095, China |
| Abstract: | Streptococcus suis type 2(SS2) is an important zoonosis agent,of which muramidase-released protein and extracellular protein factor are two important virulent factors.5 pairs of specific primers were designed and synthesized according to the sequences of MRP gene and EF gene from GenBank.4 isolates of SS2 were sequenced and analysed.All whole open reading fragment of MRP gene among 4 isolates of SS2 were 3 771 bp encoding 1 256 amino acids.All whole open reading fragment of EF gene among 4 isolates of SS2 were 2 532 bp encoding 843 amino acids.MRP showed a similary of 99.9% to those of D282 in both DNA sequence and amino acid sequence.EF exhibited the similaries of 99.9% and 99.5%-100.0% to those of D282 in DNA sequence and amino acid sequence,respectively. |
| Keywords: | Streptococcus suis type 2 MRP gene EF gene sequence analyses |
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