PCR-based detection of Toxoplasma gondii from cattle in southern Iran |
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| Institution: | 1. Parasitology Dept, Jahrom University of Medical Sciences, Jahrom, Iran;2. Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran;3. Research center for health Sciences, Institute of Health, Department of Vector Biology and Control of Diseases, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran;4. Research Centre for Non-Communicable Diseases, Department of Advanced Medical Sciences and Technologies, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran;5. Jahrom City Veterinary Office, Jahrom, Iran;1. Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan;2. Racehorse Clinic, Tokyo Racecourse, Japan Racing Association, 1-1 Hiyoshi-cho, Fuchu, Tokyo, 183-0024, Japan;1. Department of Bacteriology, Animal Health Research Institute, Ismailia lab, First District, Ismailia 41522, Egypt;2. Laboratory of Veterinary Microbiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan;3. Department of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Suez Canal University, Ring road, Ismailia 41522, Egypt;4. Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo 183–8509, Japan;5. Department of Bacteriology, Animal Health Research Institute, P.O. Box 264, Giza 12618, Egypt;6. Department of Virology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt;7. Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ring road, Ismailia 41522, Egypt;1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;3. Department of Medical Parasitology and Mycology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran;4. School of Biology, College of Science, University of Tehran, Tehran, Iran;1. Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil;2. Médico Veterinário, Auditor Fiscal Agropecuário, Agência de Defesa e Inspeção Agropecuária do Estado do Amapá, AP, Brazil;3. Laboratório de Biotecnologia Medicinal, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Macapá, AP, Brazil;4. Curso de Medicina Veterinária, Universidade Santo Amaro, São Paulo, Brazil;1. Department of Veterinary Medicine, Institute of Applied Science, Ho Chi Minh City University of Technology (HUTECH), 475A Dien Bien Phu Street, Ward 25, Binh Thanh District, Ho Chi Minh City 700000, Viet Nam;2. NTT High-Tech Institute, Nguyen Tat Thanh University, 298-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh City 700000, Viet Nam |
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| Abstract: | ObjectiveToxoplasma gondii is a protozoan parasite that is widely prevalent in most warm-blooded vertebrates. Humans mainly become infected by eating raw or undercooked meat. This study was designed to investigate the infection of cattle with T. gondii in Jahrom, southern Iran.MethodsTissue samples consisting of heart, diaphragm, and tongue were collected from 125 slaughtered cattle. DNA samples were extracted from the homogenized tissues. T. gondii was detected and genotyped using nested-polymerase chain reaction (Nested-PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based on GRA6 and SAG2 (3', 5' terminal regions) genes, respectively.ResultsThe prevalence of T. gondii DNA was 56% in cattle. The most infected tissue was the diaphragm (54.4%) followed by the heart (48.8%) and tongue (43.2%). Type II was the most prevalent genotype (70%) among T. gondii isolates.ConclusionIn this study, the high prevalence of T. gondii infection in cattle meat indicates the important role of cattle in the transmission of infection to humans. Therefore, incorporating the correct method of consuming meat in health education programs is crucial to prevent human infection. |
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| Keywords: | PCR genotyping cattle Jahrom Iran |
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