Flea-borne pathogens in the cat flea Ctenocephalides felis and their association with mtDNA diversity of the flea host |
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| Institution: | 1. Higher Institution Centre of Excellence (HICoE), Tropical Infectious Diseases Research & Education Centre (TIDREC), Universiti Malaya, 50603, Kuala Lumpur, Malaysia;2. Institute for Advanced Studies, Universiti Malaya, 50603, Kuala Lumpur, Malaysia;3. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia;4. Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia;1. Imunova Análises Biológicas, Setor de Ciências Biológicas UFPR, Curitiba, PR, 81531-980, Brazil;2. Universidade Federal do Paraná, Setor de Ciências Biológicas UFPR, Curitiba, 81531-980, Brazil;3. Zoetis Indústria de Produtos Veterinários Ltda, São Paulo, SP, 04709-111, Brazil;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Microbiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania;2. Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary;3. Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07, Uppsala, Sweden;4. SLU Global Bioinformatics Centre, Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07, Uppsala, Sweden;5. Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, 1425, Ciudad Autónoma de Buenos Aires, Argentina;6. Instituto de Agrobiotecnología y Biología Molecular, IABiMo, INTA-CONICET, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, Castelar, 1712, Buenos Aires, Argentina;1. Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland;2. Institut Central des Hôpitaux Valaisans, Infectious Diseases, Sion, Switzerland;3. Institut Pasteur, Alger, Algeria;1. École Nationale Supérieure Vétérinaire d’Alger, El Harrach, Alger, Algeria;2. Université Paris-Est, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, ENVA, Anses, USC INRA, Avenue du général de Gaulle, F94704 Maisons-Alfort, France;3. INRA, Maisons-Alfort, France;1. Comité Nacional de Investigaciones Científicas y Técnicas (CONICET), Ministerio de Ciencia, Tecnología e Innovación Productiva, Buenos Aires, Argentina;2. Instituto Nacional de Medicina Tropical (INMeT), ANLIS, Ministerio de Salud y Desarrollo Social de la Nación, Jujuy y Neuquén s/n, 3370, Puerto Iguazú, Misiones, Argentina;3. Departamento de Parasitologia, Instituto de Ciéncias Biológicas, Universidade Federal de Minas Gerais, Av. Pres. A. Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil;4. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) (CONICET CCT La Plata-UNLP), Boulevard 120 s/n e/60 y 64, 1900, La Plata, Argentina |
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| Abstract: | Flea-borne pathogens were screened from 100 individual cat fleas using a PCR approach, of which 38 % were infected with at least one bacterium. Overall, 28 % of the flea samples were positive for Bartonella as inferred from ITS DNA region. Of these, 25 % (7/28) were identified as Bartonella clarridgeiae, 42.9 % (12/28) as Bartonella henselae consisted of two different strains, and 32.1 % (9/28) as Bartonella koehlerae, which was detected for the first time in Malaysia. Sequencing of gltA amplicons detected Rickettsia DNA in 14 % of cat flea samples, all of them identified as Rickettsia asembonensis (100 %). None of the flea samples were positive for Mycoplasma DNA in 16S rRNA gene detection. Four fleas were co-infected with Bartonella and Rickettsia DNAs. Statistical analyses reveal no significant association between bacterial infection and mtDNA diversity of the cat flea. Nevertheless, in all types of pathogen infections, infected populations demonstrated lower nucleotide and haplotype diversities compared to uninfected populations. Moreover, lower haplotype numbers were observed in infected populations. |
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| Keywords: | Flea Pathogens Association Genetic diversity |
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