A rapid chronometric assay of peroxidase activity in citrus leaf discs |
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| Authors: | A Torrecillas A Leon F Del Amor MC Martinez-Mompean |
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| Institution: | E.I. de Nutrición, Fertilización y Riego, Centro de Edafologia y Biologǐa Aplicada del Segura (CSIC), P.O. Box 195, 30003 Murcia Spain |
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| Abstract: | A rapid peroxidase assay is proposed, using 3-mm diameter leaf discs, and based on the chronometric measurement of the oxidation of ascorbic acid, which is detected by means of the appearance of the blue colour of the benzidine-coupled reaction.The proposed method is adequately reproducible and sensitive for the diagnosis of iron deficiency in citrus. |
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| Keywords: | citrus iron peroxidase |
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