Structure of the Cochliobolus sativus population variability |
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| Authors: | L Leisova‐Svobodova V Minarikova L Kucera S A Pereyra |
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| Institution: | 1. Department of Molecular Biology, Crop Research Institute Prague, Drnovska 507, Prague 6 – Ruzyne, 161 06;2. Agricultural Research Institute, Havlickova 2787, 767 07 Kromeriz, Czech Republic;3. Plant Pathology, INIA La Estanzuela, Ruta 50 CC39173, 70000 Colonia, Uruguay |
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| Abstract: | The genetic diversity of several local populations of the fungal pathogen Cochliobolus sativus, collected over 3 years from different regions of the Czech Republic, was examined using amplified fragment length polymorphism (AFLP). A high level of variability was found even among isolates from one lesion. Measures of multilocus linkage disequilibrium suggested that recombination has a minor impact on the genetic structure of populations. Cochliobolus sativus forms genetically divergent populations (FST = 0·33), indicating a low level of geneflow between populations. This was supported by a significant correlation between genetic diversity and geographical distances up to 80–100 km. The most likely explanation for the genetic variability is that the fungus forms conidia with highly variable chromosomal rearrangements. The differentiation observed among local populations implies that genetic drift, including a founder effect, combined with restricted migration generates the structure of C. sativus populations. |
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| Keywords: | amplified fragment length polymorphism Cochliobolus sativus diversity geneflow index of association linkage disequilibrium |
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