Phenylalanine ammonia‐lyase and cinnamate 4‐hydroxylase genes' response to HHO in Eupatorium adenophorum |
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| Authors: | Xixiang Pei Fanghao Wan Hongmei Cheng |
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| Institution: | 1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, , Beijing, China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, , Beijing, China |
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| Abstract: | 6‐Hydroxy‐5‐isopropyl‐3,8‐dimethyl‐4a,5,6,7,8,8a‐hexahydronaphthalen‐2(1H)‐one (HHO) is an allelopathic substance that is related to allelochemical metabolism in Eupatorium adenophorum. In this study, the full‐length complementary DNA of two phenylalanine ammonia‐lyase (PAL) genes and a fragment of the cinnamate 4‐hydroxylase (CA4H) gene from E. adenophorum (Ea) were cloned. The expression of all three genes was increased by the HHO treatment, with varying sensitivity: EaPAL2 > EaPAL1 > EaCA4H. Southern blotting suggested that there is a third member of the PAL gene family in this species. |
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| Keywords: | cinnamate 4‐hydroxylase Eupatorium adenophorum HHO phenylalanine ammonia‐lyase |
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