Osmoregulatory ability and stress responses during freshwater adaptation of black porgy (Acanthopagrus schlegeli) treated with exogenous prolactin |
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| Authors: | Mi Seon Park Byung Hwa Min Tae Seok Moon Han Kyu Lim Cheol Young Choi Young Jin Chang Kang Hee Kho |
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| Institution: | 1. East Sea Fisheries Research Institute, National Fisheries Research & Development Institute, , Gangneung, South Korea;2. Department of Aquaculture Management, National Fisheries Research & Development Institute, , Busan, South Korea;3. Division of Marine Environment & Bioscience, Korea Maritime University, , Busan, South Korea;4. Department of Marine Bio‐Materials & Aquaculture, Pukyong National University, , Busan, South Korea;5. Department of Marine Technology, College of Fisheries & Ocean Science, Chonnam National University, , Yosu, South Korea |
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| Abstract: | The effects of ovine prolactin (oPRL) on osmoregulatory ability (electrolyte balance, plasma osmolality and activity of gill chloride cells and gill Na+/K+‐ATPase) and stress responses (plasma cortisol, glucose, aspartate aminotransferase: AST and alanine aminotransferase: ALT) were investigated in black porgy transferred to freshwater (FW). Fish in seawater (SW) were injected twice at a 24 h interval with oPRL (at 1, 3, or 5 μg g–1 body weight) or vehicle (0.9% NaCl) and then transferred to FW. They were sampled 3 days after the transfer. With oPRL at 5 μg g–1, levels of plasma Na+ and Cl? and osmolality were significantly higher than in saline‐treated fish, whereas gill CCs number and Na+/K+‐ATPase activity were lower. Also, the 5 μg g–1oPRL treatment led to significantly lower plasma cortisol levels than did saline treatment. However, there were no significant differences in plasma AST and ALT between groups. These results support the positive osmoregulatory role of PRL in black porgy during FW adaptation. |
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| Keywords: | black porgy freshwater adaptation osmoregulation stress response exogenous prolactin |
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