Effects of erythromycin and its derivatives on the proliferation of T lymphocyte in vitro |
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| Authors: | WU Lan ZHANG Wei-ge ZHANG Chuan-liang QU Ying-wei ZHAO Li-juan |
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| Institution: | 1.Department of Geriatrics, 3 Departent of Rheumatology and Immunology, The First Affiliated Hospital of Chinese Medical University, Shenyang 110001, China;2.Department of Pharmaceutical Chemistry, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. E-mail: zhaolijun@21cn.com |
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| Abstract: | AIM: To study the effects of erythromycin and its derivatives on the proliferation and the apoptosis of T lymphocytes and to evaluate the anti-inflammatory mechanism of erythromycin derivatives. METHODS: The effects of four erythromycin derivatives without antibacterial action on the proliferation T lymphocytes were evaluated by MTT. The effects of the erythromycin derivatives on apoptosis in T lymphocytes were observed by flow cytometry and TUNEL staining. RESULTS: The proliferation of T lymphocytes were inhibited by erythromycin derivative-Ⅰ and erythromycin, the IC50 was (425.2±32.1) μmol/L and (606.3±35.4) μmol/L, respectively. The IC50 value of erythromycin derivative-Ⅰ was lower than that of EM (P<0.01). Erythromycin derivative-Ⅰ (3-30 mg/L) and EM (30-100 mg/L) induced T lymphocytes apoptosis in a concentration-related manner by flow cytometry and TUNEL, while erythromycin derivative-Ⅰ induced cells to die at concentration of 100 mg/L. CONCLUSION: The anti-inflammatory activity of erythromycin derivatives may be due partly to their effects on the proliferation and apoptosis of T lymphocytes. |
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| Keywords: | Erythromycin T-lymphocytes Apoptosis |
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