Role of bcl-2 gene expression in inhabition of hepatocellular carcinoma by genistein in nude mice |
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| Authors: | ZHANG Ji-hong LIANG Li-jian HUANG Jie-fu |
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| Institution: | 1.Division of Hepato-biliary Surgery, 2Clinical College and The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China;3.Division of Hepato-biliary Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.E-mail:zhjihong@hotmail.com |
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| Abstract: | AIM:To probe into the role of 1, 4, 5 - trisphosphate inositol (IP3) and bcl-2 gene expression in inhibiting hepatocellular carcinoma of nude mice by genistein. METHODS:Animals with hepatocellular carcinoma were treated with genistein 1 mg·kg-1·d-1 (ip) for 3 weeks. The volume and weight of tumaor were measured. IP3, bcl-2 mRNA, Bcl-2 protein were assayed by IP3-［3H］ Birtrak assay, RT-PCR, Western blotting, respectively. RESULTS:The tumor volume and weight of animals treated with genistein were lower than those in control (42.7mm3±27.8mm3 vs 52.3mm3±26.5mm3, 42.7mg±27.8 mg vs 91.3mg±31.4 mg). IP3 content was lower than that in control ［(13.4±1.4)nmol/g protein vs (35.3±6.6)nmol/g protein］. bcl-2 mRNA expression was lower in group treated with genistein than that in control (RI which was the gray degree multiply area of bcl-2 / the gray degree multiply area of β-actin 0.48±0.02 vs 0.56±0.15). Bcl-2 protein expression was lower in group treated with genistein than that in control (RI 1.69±0.52 vs 1.37±0.48). CONCLUSION:Genistein inhibits growth of transplanted hepatocellular carcinoma in nude mouse liver by reducing IP3 production and down-regulating bcl-2 gene expression. |
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