Effects of endoplasmic reticulum stress induced by increased expression of calcium-sensing receptor in myocardial hypoxia/re-oxygenation injury |
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| Authors: | SUN Zhi-rui WANG Yan-li DU Li-juan ZHAO Ya-jun WANG Li-na XU Chang-qing ZHANG Wei-hua |
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| Institution: | Department of Pathophysiology, Harbin Medical University, Harbin 150086, China. E-mail: zhangwh116@hotmail.com |
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| Abstract: | AIM: To observe the effects of endoplasmic reticulum stress induced by the increase in expression of calcium-sensing receptor in myocardial hypoxia/re-oxygenation injury. METHODS: The primarily neonatal rat ventricular cardiomyocytes were incubated for 4-5 d, then randomly divided into 5 groups: (1) sham control group; (2) hypoxia/re-oxygenation group; (3) H/Re+ NiCl2, CdCl2-reperfusion group; (4) H/Re+GdCl3, NiCl2, CdCl2-reperfusion group; (5) H/Re+caffeine,GdCl3, NiCl2, CdCl2-reperfusion group. The neonatal cells were in ischemia-mimetic solution for 3 h, and re-incubated cells in normal culture medium for 9 h to establish a model of H/Re. The activity of LDH was determined, the viability and apoptosis of cardiomyocytes were assayed by MTT, the expressions of CaSR and caspase-12 in each group were analyzed using Western blotting, and the concentration of intracellular calcium was measured by laser confocal scanning microscope. RESULTS: The apoptosis index, the activity of LDH, the concentration of intracellular calcium, and quantitative expression of CaSR and caspase-12 in H/Re and activator groups were significantly higher than those in control group, while the viability and apoptosis of cardiomyocytes were lower than those in control group. CONCLUSION: In myocardial hypoxia/re-oxygenation, CaSR induces endoplasmic reticulum stress by altering the intracellular calcium homeostasis. The induction of apoptosis may be due to the increase in the expreesions of caspase-12 and other proapoptotic proteins. |
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