Role of cyclin D1 in PKC regulating the proliferation of airway smooth muscle cells in asthmatic rat |
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| Authors: | QIAO Li-fen XU Yong-jian LIU Xian-sheng XIE Jun-gang DU Chun-ling ZHANG Jian NI Wang CHEN Shi-xin |
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| Institution: | Department of Respiratory Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. E-mail:yjxu@tjh.tjmu.edu.cn |
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| Abstract: | AIM: To observe the expression of cyclin D1 following PKC activation and the correlation between PKC-α and cyclin D1 in asthmatic rat airway smooth muscle cells (ASMCs), and to discuss the effects of cyclin D1 on the proliferation of airway smooth muscle cells regulated by PKC in asthmatic rat. METHODS: Twelve SD rats were randomly divided into control group (group N) and asthmatic 2-week group (group A), and then subdivided into 4 groups based on the drug interventions: (1) control group; (2) PMA; (3) PMA+5 μmol/L Ro-31-8220 group; (4) 5 μmol/L Ro-31-8220 group. The proliferations of ASMCss were examined with cell cycle analysis, MTT colorimetric assay and proliferating cell nuclear antigen (PCNA) immunocytochemical staining. The expressions of PKC-α and cyclin D1 were analyzed by RT-PCR and Western blotting. The data were subject to correlation analysis. RESULTS: (1) Compared to group A1, there were significant differences in the percentage of S+G2/M phase, absorbance A value of MTT and the rate of positive expression of PCNA protein in group A2 and A4 (P<0.01). The same tendency in group N was observed according with group A. (2) Compared with A1, the ratios of A values of PKC-α mRNA and protein in group A2 and A4 were significantly changed (P<0.01) as well as the ratios of A values of cyclin D1 mRNA and protein. (3) There were positive correlations between PKC-α and cyclin D1 in mRNA (r=0.476, P<0.05) and protein(r=0.899, P<0.01). CONCLUSION: The activation of PKC-α promotes ASMCs proliferation in asthmatic rats, and there are positive correlations between the PKC-α and cyclin D1 in mRNA and protein, indicating that the PKC-α signal pathway may be involved in the process of airway smooth muscle remodeling by regulating the expression of cyclin D1 in asthmatic rats. |
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