| Al3+对大豆根边缘细胞释放与活性的影响 |
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| 引用本文： | 邢承华,蔡妙珍,刘鹏,徐根娣,蔡慧姝,章月琴.Al3+对大豆根边缘细胞释放与活性的影响[J].植物营养与肥料学报,2008,14(3):597-601. |
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| 作者姓名： | 邢承华 蔡妙珍 刘鹏 徐根娣 蔡慧姝 章月琴 |
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| 作者单位： | 1.金华职业技术学院生物工程学院,浙江金华321007; |
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| 基金项目： | 国家自然科学基金
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浙江省自然科学基金 |
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| 摘 要： | 以两个大豆 Glycine max (L.) Merr.] 品种（耐铝性大豆浙春2号和铝敏感性大豆华春18）的边缘细胞为材料，比较研究了Al3+对根尖原位边缘细胞释放以及对离体边缘细胞的毒害作用。结果显示，0、100、200 μmol/L Al3+处理后，浙春2号和华春18的根尖边缘细胞在水中均易分散，华春18的边缘细胞在300 μmol/L的Al3+处理时已聚集成团不易分散，而浙春2号的边缘细胞在400 μmol/LAl3+处理时才不易分散。Al3+对离体边缘细胞有明显的毒害作用，100 μmol/L Al3+处理1～ 6 h就表现出细胞死亡症状，毒害作用最大时出现在6 h之后，其中Al3+对华春18的毒害作用略高于浙春2号。系列浓度Al3+ （0、100、200、300、400 μmol/L Al3+）处理，100 mol/ L Al3+处理的离体边缘细胞存活率已出现较大幅度的下降，至400 mol/L Al3+处理时，浙春2号和华春18的相对存活率分别只有对照的45.9%和39.0%。 说明外界Al3+浓度升高不仅影响边缘细胞的释放，而且显著降低离体边缘细胞的存活率，毒害作用最大时出现在6 h之后。大豆品种间细胞对Al3+的反应存在一定差异，随时间变化，差异最大时在6 h。
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| 关 键 词： | 大豆 边缘细胞 Al3+毒害 释放 细胞活性 |
| 文章编号： | 1008-505X(2008)03-0597-05 |
| 收稿时间： | 2007-5-14 |
| 修稿时间： | 2007年5月14日 |
Effect of Al3+toxicity on release and viability of soybean root border cells |
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| XING Cheng-hua,CAI Miao-zhen,LIU Peng,XU Gen-di,CAI Hui-shu,ZHANG Yue-qin.Effect of Al3+toxicity on release and viability of soybean root border cells[J].Plant Nutrition and Fertilizer Science,2008,14(3):597-601. |
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| Authors: | XING Cheng-hua CAI Miao-zhen LIU Peng XU Gen-di CAI Hui-shu ZHANG Yue-qin |
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| Institution: | 1.Bioengineering Institute,Jinhua Vocational and Technological College,321007 Jinhua,China; |
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| Abstract: | Experiments were carried out to examine Al3+ effects on release of border cells in situ around root tips or in suspension by comparing responses of two soybean ［Glycine max (L.) Merr.］ cultivars (Al-resistance Zhechun No. 2 and Al-sensitive Huachun No.18) known to differ in Al resistance at the whole-root level. Both cultivars' border cells in situ exposed to 0, 100, 200 μmol/L Al3+ dispersed readily into suspension, however, border cells of Huachun No.18 and roots clumped together and did not dispense at 300 μmol/L and 400 μmol/L Al3+, respectively. Al3+ treatment significantly decreased cell viability, cell death was found during a 1-6 h Al3+ exposure time at a concentration of 100 μmol/L, and the cell death was greatest after 6 h Al3+ treatment. Toxic effect on cell viability was higher in Huachun No.18 than that of Zhuchun No.2 during the whole suspension culture. Cell survival percentage of border cells in vitro dropped greatly when treated with 100 μmol/L Al3+. When the treatment level increased to 400 μmol/L Al3+, cell survival percentage of Zhechun No. 2 and Huachun No.18 were only 31.6% and 26.2%, respectively. These results indicate that Al3+ solution may affect release of border cells from root tip, and significant reduce their survival rate, with the highest toxicity effect occurring after 6 h. These responses were different with cultivars, and the differences were largest at 6 h after treatment. This Al-resistance mechanism by releasing border cells may act as one of the purported conditioning factors thought to affect the vitality of in vitro cultures. |
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