| 水涝胁迫下海滨锦葵幼苗根尖细胞内Ca~(2+)分布与变化 |
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| 引用本文： | 周建,钦佩.水涝胁迫下海滨锦葵幼苗根尖细胞内Ca~(2+)分布与变化[J].东北林业大学学报,2016(3):61-67. |
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| 作者姓名： | 周建 钦佩 |
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| 作者单位： | 1. 河南科技学院，新乡，453003;2. 南京大学 |
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| 基金项目： | 河南省教育厅自然科学项目，河南科技学院2014年校攀登计划 |
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| 摘 要： | 采用醋酸双氧铀染色与透射电镜技术,以海滨锦葵为试验材料,连续观测水涝胁迫下及涝后恢复期间海滨锦葵根尖细胞内Ca~(2+)的分布与变化特性。结果显示:随着水涝时间延长,海滨锦葵根尖细胞间隙与细胞核、液泡中钙离子沉积密度逐步降低,质体外膜上存在Ca~(2+)分布,但低于对照组,而Ca~(2+)向细胞质移动,在局部区域聚集,导致细胞质钙离子增加。水涝去除20 d后,细胞壁中出现钙离子沉积,细胞间隙、质体外膜上与液泡中所分布钙离子增加,细胞质所聚集的Ca~(2+)逐步分散,基本不存在Ca~(2+)沉积。研究认为,水涝胁迫下,海滨锦葵根尖细胞内Ca~(2+)浓度迅速上升;涝后恢复期间,Ca~(2+)浓度逐渐下降,起着外界信号传递的作用。
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| 关 键 词： | 水涝胁迫 海滨锦葵 根尖细胞 Ca2%2B 离子分布 |
Distribution and Variation of Ca2+in Root Apical Cells of Kosteletzkya virginica (L.) Seedlings under Waterlog-ging |
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| Abstract: | With Kosteletzkya virginica ( L.) , we observed its distribution and variation of Ca 2+in root apical cells continuously by transmission electron microscope combined with uranyl acetate staining method in and after waterlogging stress .During wa-terlogging, Ca2+of root apical cells mainly distributed within intercellular spaces , nuclei and vacuoles , but the density of Ca2+reduced gradually .Additionally,there were calcium ions located on outer membranes of plastids , but its density was lower than that in control within this stress .However, Ca2+showed a trend to move into cytoplasm , and accumulated to in some areas .On the 20 th day after drainage of waterlogging , the amassed calcium ions released , and almost no Ca 2+distrib-uted in cytoplasm .However , with the release of cytoplasm Ca 2+, the densities of calcium ions were increased in intercellu-lar space, vacuoles and outer membrane of plastid , a great deal of Ca 2+located with cell walls in recovery period .After 20 days of recovery , the distribution of Ca 2+in waterlogged plants was similar to that in control ones .The density of Ca 2+in root apical cell was increased quickly in waterlogging and subsequently decreased in restoration , and Ca2+not only affected cell function, but also acted as a means to transmit outside information in K.virginica plants. |
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| Keywords: | Waterlogging stress Kosteletzkya virginica(L ) Root apical cell Ca2+ Ion distribution |
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