The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development |
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| Authors: | Qiao-Chu WANG Jun LIU Xing DUAN Xiang-Shun CUI Nam-Hyung KIM Bo XIONG Shao-Chen SUN |
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| Institution: | 1)College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;2)Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Korea |
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| Abstract: | Dynamin 2 is a large GTPase notably involved in clathrin-mediated endocytosis, cell migration and cytokinesis in mitosis. Our previous study identified that Dynamin 2 regulated polar body extrusion in mammalian oocytes, but its roles in early embryo development, remain elusive. Here, we report the critical roles of Dynamin 2 in mouse early embryo development. Dynamin 2 accumulated at the periphery of the blastomere during embryonic development. When Dynamin 2 activity was inhibited by Dynasore, embryos failed to cleave to the 2-cell or 4-cell stage. Moreover, the actin filament distribution and relative amount were aberrant in the treatment group. Similar results were observed when embryos were cultured with Dynasore at the 8-cell stage; the embryos failed to undergo compaction and develop to the morula stage, indicating a role of Dynamin 2 in embryo cytokinesis. Therefore, our data indicate that Dynamin 2 might participate in the early embryonic development through an
actin-based cytokinesis. |
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| Keywords: | Actin filament Cytokinesis Dynamin Embryo |
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