Anthocyanin characterization utilizing liquid chromatography combined with advanced mass spectrometric detection |
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| Authors: | Steimer Sarah Sjöberg Per J R |
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| Institution: | Department of Physical and Analytical Chemistry, Uppsala University, Box 599, SE-751?24 Uppsala, Sweden. |
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| Abstract: | Anthocyanins are naturally occurring polyphenolic plant pigments. To analyze the anthocyanin content of samples, rapid and reliable methods for separation and detection are needed. In this work an LC-DAD-ESI-MS/MS instrument was used to develop a new tandem MS data acquisition strategy for anthocyanin characterization which was subsequently compared to more conventional measurements. It has been shown that the newly developed strategy, multiple reaction monitoring-initiated anthocyanin characterization (MIAC), can successfully be used in anthocyanin analysis and has various advantages compared to some more traditional measurements, such as enhanced selectivity, better signal-to-noise ratio and simplified data evaluation. Furthermore, the number of relevant MS/MS data increased significantly with the MIAC method compared with a more common information dependent MS experiment strategy. |
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