| 拟南芥高迁移率族蛋白B(HMGB)族基因的克隆及表达分析 |
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| 引用本文： | 冀芦沙,郭尚敬.拟南芥高迁移率族蛋白B(HMGB)族基因的克隆及表达分析[J].安徽农业科学,2010,38(35):19929-19932. |
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| 作者姓名： | 冀芦沙 郭尚敬 |
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| 作者单位： | 聊城大学生命科学学院,山东聊城,252059;聊城大学生命科学学院,山东聊城,252059 |
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| 摘 要： | 目的]为研究高等植物高迁移率族蛋白B家族的功能及作用模式。方法]运用RT-PCR方法克隆出拟南芥HMGB蛋白家族中的3个基因：At2G34450、At5G23405、At5G23420,并运用SDS-PAGE、Northern检测及亚细胞定位等手段检测这3种蛋白在大肠杆菌及拟南芥中的表达。结果]经鉴定,以上3种蛋白的分子量分别为17.5、17.0和27.5 kD,在拟南芥中表达量At5G23420〉At5G23405〉At2G34450,且这3种蛋白均定位于细胞核内。结论]该研究结果为深入研究高等植物中HMGB家族蛋白的生物学功能奠定了基础。
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| 关 键 词： | 拟南芥高迁移率族蛋白B家族(AtHMGB) 原核表达 亚细胞定位 Northern检测 |
The Cloning and Expression Analysis of the High Mobility B Group Genes in Arabidopsis thaliana |
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| Institution: | JI Lu-sha et al(School of Life Science,Liaocheng University,Liaocheng,Shandong 252059) |
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| Abstract: | Objective] The aim was to better research the function and action mode of High Mobility Group B(HMGB) proteins in higher plants.Method] At2G33450,At5G23405 and At5G23420 genes of HMGB protein family in Arabidopsis thaliana were cloned by the use of RT-PCR method,and the expression of these three proteins in E.coli and Arabidopsis thaliana were detected by using SDS-PAGE,Northern blot and subcellular localization methods.Result] The results showed that the molecular weights of the three protein were 17.5,17.0 and 27.0 kD respectively,and the expression levels of the proteins in Arabidopsis thaliana were At5G23420>At5G23405>At2G33450.In addition,all the three proteins were located in nucleus.Conclusion] The study will provide a basis for the further research on the biological function of HMGB proteins in higher plants. |
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| Keywords: | Arabidopsis thaliana High Mobility Group B(AtHMGB) Prokaryotic expression Subcellular localization Northern blot |
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