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珍珠拳组培快繁技术研究初报
引用本文:曾少玲,方良,全吉文,郭欣.珍珠拳组培快繁技术研究初报[J].广东林业科技,2002,18(2):15-18.
作者姓名:曾少玲  方良  全吉文  郭欣
作者单位:广东省湛江市林业科学研究所,湛江,524037
摘    要:通过播种获得外植体无菌材料,进行了珍珠拳组培快繁技术研究。结果表明:MS基本培养基适合分化,加入6BA0.5mg/L适合培殖培养,加AD4.0mg/L使芽活力明显提高,加多效唑5.0mg/L对芽有较好的矮化效果。white基本培养基适合生根,其生根培养以IBA1.0mg/L+NAA0.5mg/L为最佳激素组合。

关 键 词:珍珠拳  组织培养  培养基  快速繁质技术  激素组合

TISSUE CULTURE AND RAPID PROPAGATION OF SECURINEGA SUFFRUTICOSA
Zeng Shaoling,Fang Liang,Quan Jiwen,Guo Xin.TISSUE CULTURE AND RAPID PROPAGATION OF SECURINEGA SUFFRUTICOSA[J].Forestry Science and Technology of Guangdong Province,2002,18(2):15-18.
Authors:Zeng Shaoling  Fang Liang  Quan Jiwen  Guo Xin
Abstract:This paper studied the major process of tissue culture and rapid propagation of Securinega suffruticosa (Pall.) Rehd. for the asepsis materials from seeds. Among the basic culture medium,the MS culture medium is fit to differentiation, and the white culture medium is fit for takeing root. In the successive transfer culture process, 0.5 mg/L 6BA and 4.0 mg/L AD content added was apparenthy benefit to enhance bud quality, and yet 5.0 mg/L paclobutrazor added make bud good dwarfing. 1.0 mg/L IBA + 0.5 mg/L NAA was content constituting for rooting.
Keywords:Securinega suffruticosa      tissue culture      culture medium  
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