| 淡紫拟青霉几丁质酶的纯化和活性影响因子 |
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| 引用本文： | 肖炎农,王明祖.淡紫拟青霉几丁质酶的纯化和活性影响因子[J].植物病理学报,1998,28(3):275-280. |
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| 作者姓名： | 肖炎农 王明祖 |
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| 作者单位： | 华中农业大学植保系, 武汉 430070 |
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| 摘 要： | 淡紫拟青霉(Paecilomyces lilacinus)是一种重要的植物线虫卵寄生真菌。采用DEAE-22离子交换层析和SephcrylS-300凝胶过滤层析分离纯化到一种淡紫拟青霉胞外几丁质酶,经7.5%聚丙烯酰胺凝胶电脉检测为单一条酶带,Rf值等于0.43。淡紫拟青霉几丁质酶的最适温度为45℃。热稳定性测定表明它在40℃下放置10min可保持原有活力,60℃下10min,完全失活。该酶的最适pH值为5。其pH值稳定性与温度和反应时间有关。20℃、20min,在pH3~9范围内稳定;37℃、1h,则只在pH5~7范围内较稳定。金属离子如Mg2+、Ca2+、Zn2+、Li+和Fe2+对酶有一定的激活作用,其中Mg2+的激活作用最强。相反,Cu2+对酶有强烈抑制作用。
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| 关 键 词： | 淡紫拟青霉 几丁质酶 分离纯化 活性影响因子 |
PURIFICATION OF EXTRACELLULAR CHITINASE PRODUCED BY PAECILOMYCES LILACINUS AND ITS ACTIVITY INFLUENCING FACTORS |
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| Xiao Yannong,Wang Mingzu,Wang,Daoben,Hu Xiaohui.PURIFICATION OF EXTRACELLULAR CHITINASE PRODUCED BY PAECILOMYCES LILACINUS AND ITS ACTIVITY INFLUENCING FACTORS[J].Acta Phytopathologica Sinica,1998,28(3):275-280. |
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| Authors: | Xiao Yannong Wang Mingzu Wang Daoben Hu Xiaohui |
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| Institution: | Department of Plant Protection, Huazhong Agricultural University, Wuhan 430070 |
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| Abstract: | A chitinase from culture filtrate of Paecilomyces lilacinus, an important parasite of plant nematode eggs, was purified by preciptation with ammonium sulfate followed by ion exchange chromatography on DEAE-22 and gel filtration on Sephcryl S-300. The optimum pH for the enzyme was pH 5, and maximum activity was obtained at 45℃. for 10 minutes and denatured at 60℃ for 10 minutes. It was comparatively stable within pH 3~9 at 20℃ for 20 minutes and within pH 5~7 at 37℃ for one hour, Metallic ions such as Mg2+, Ca2+, Fe2+,Zn2+ and Li+ could activate the enzyme. |
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| Keywords: | Chitinase Paecilomyces lilacilus Activity variation Purification Extraction |
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