| 水稻细菌性条斑病抗性QTL qBlsr5a的精细定位 |
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| 引用本文： | 韩庆典,陈志伟,邓云,兰涛,官华忠,段远霖,周元昌,林闽川,吴为人.水稻细菌性条斑病抗性QTL qBlsr5a的精细定位[J].作物学报,2008,34(4):587-590. |
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| 作者姓名： | 韩庆典 陈志伟 邓云 兰涛 官华忠 段远霖 周元昌 林闽川 吴为人 |
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| 作者单位： | 福建农林大学作物遗传改良研究所, 福建福州350002 |
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| 摘 要： | 分别以高抗细菌性条斑病的品种Acc8558和高感的品种H359为供体和受体亲本, 通过回交和分子标记辅助选择, 育成了只渗入5号染色体短臂上单个供体亲本细条病抗性QTL(qBlsr5a)的近等基因系H359-BLSR5a。将该近等基因系与H359杂交, 建立了一个大的F2群体。采用选择极端表型个体并验证其后代(F2:3)株系的方法, 在F2群体中鉴定出目标QTL为抗病纯合基因型的个体。通过对这些个体进行分子标记基因型检测和连锁分析, 将qBlsr5a定位在SSR标记RM153和RM159之间, 大约2.4 cM或290 kb的范围内。
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| 关 键 词： | 水稻细菌性条斑病 QTL 精细定位 |
| 收稿时间： | 2007-09-06 |
| 修稿时间： | 1900-01-01 |
Fine Mapping of qBlsr5a, a QTL Controlling Resistance to Bacterial Leaf Streak in Rice |
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| Institution: | Institute of Crop Genetic Improvement, Fujian Agriculture & Forestry University, Fuzhou 350002, Fujian, China |
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| Abstract: | Bacterial leaf steak (BLS) is one of the most destructive diseases in rice. Using a recombinant inbred line population derived from a cross between a highly-resistant variety Acc8558 and a highly-susceptive variety H359, 11 QTLs controlling rice resistance to BLS were previously mapped, among which a QTL on the short arm of chromosome 5 named qBlsr5a had the largest effect. This QTL was further verified and more precisely mapped later. In this study, with Acc8558 as the donor and H359 as the recipient, an near-isogenic line H359-BLSR5a was created by backcross breeding with marker-assisted selection, in which only the QTL qBlsr5a was introgressed from the donor parent. A large F2 population was constructed by hybridizing the near-isogenic line with H359. By selecting individuals with extreme phenotypes and examining their progeny (F2:3) lines, individuals with the resistant homozygous genotype at the target QTL in the F2 population were identified. By genotyping these individuals with SSR markers and performing linkage analysis, qBlsr5a was mapped to an interval between SSR markers RM153 and RM159, covering a range of 2.4 cM or 290 kb. |
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| Keywords: | Rice bacterial leaf streak QTL Fine mapping |
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