| 中国小麦育成品种和农家种中慢锈基因Lr34/Yr18的分子检测 |
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| 引用本文： | 杨文雄,杨芳萍,梁丹,何中虎,尚勋武,夏先春.中国小麦育成品种和农家种中慢锈基因Lr34/Yr18的分子检测[J].作物学报,2008,34(7):1109-1113. |
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| 作者姓名： | 杨文雄 杨芳萍 梁丹 何中虎 尚勋武 夏先春 |
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| 作者单位： | 1.甘肃省农业科学院作物研究所, 甘肃兰州730070;2.中国农业科学院作物科学研究所/国家小麦改良中心, 北京100081;3.安徽农业大学农学院, 安徽合肥230036;4.CIMMYT中国办事处, 北京100081;5.甘肃农业大学农学院, 甘肃兰州730070 |
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| 基金项目： | 西部之光人才培养计划
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国家科技支撑计划项目
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国家高技术研究发展计划(863计划)项目
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甘肃省牛物技术研究及应用项 |
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| 摘 要： | Lr34/Yr18是重要的慢叶锈和慢条锈基因, 携带该连锁基因的小麦品种被广泛种植于世界许多国家。利用STS标记csLV34对慢叶锈和慢条锈基因Lr34/Yr18进行分子检测的结果表明, 我国231份育成品种(系)中仅有14份材料携带Lr34/Yr18基因, 占6.1%。不同麦区分布频率不同, 其中北部冬麦区为零, 黄淮冬麦区、长江中下游冬麦区、西南冬麦区和西北春麦区分别为3.0%、21.4%、16.7%和33.3%。在422份农家种中, 359份含有Lr34/Yr18基因, 占85.1%。Lr34/Yr18基因在不同麦区的分布频率也存在差异, 北部冬麦区、黄淮冬麦区、长江中下游冬麦区、西南冬麦区、南部冬麦区和西北春麦区分别为89.6%、77.4%、93.1%、93.8%、96.6%和61.1%。csLV34标记扩增产物为150 bp和229 bp的片段, 能有效鉴别品种是否携带Lr34/Yr18基因, 是一个重复性好、准确率高的分子标记, 可用于小麦Lr34/Yr18基因的鉴定与选择。
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| 关 键 词： | 普通小麦 Lr34/Yr18基因 STS标记csLV34 分子标记辅助选择 |
| 收稿时间： | 2008-01-07 |
Molecular Characterization of Slow-Rusting Genes Lr34/Yr18 in Chinese Wheat Cultivars |
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| YANG Wen-Xiong,YANG Fang-Ping,LIANG Dan,HE Zhong-Hu,SHANG Xun-Wu,XIA Xian-Chun.Molecular Characterization of Slow-Rusting Genes Lr34/Yr18 in Chinese Wheat Cultivars[J].Acta Agronomica Sinica,2008,34(7):1109-1113. |
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| Authors: | YANG Wen-Xiong YANG Fang-Ping LIANG Dan HE Zhong-Hu SHANG Xun-Wu XIA Xian-Chun |
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| Institution: | 1.Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070 Gansu;2.Institute of Crop Sciences/ National Wheat Improve-ment Center, Chinese Academy of Agricultural Sciences, Beijing 100081;3.College of Agronomy, Anhui Agricultural University, Hefei 230036, An-hui;4.CIMMYT China Office, Beijing 100081;5.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China |
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| Abstract: | Lr34 and Yr18 are important slow rusting resistance genes that have been widely used in many countries. The aim of this study was to identify Lr34/Yr18 genes in 231 Chinese wheat cultivars and 422 landraces using an STS marker csLV34, which can amplify a 150-bp and a 229-bp fragment in the genotypes with and without Lr34/Yr18, respectively. The results indicated that only 14 genotypes carried the resistance genes Lr34/Yr18 in the improved wheat cultivars, with a frequency of 6.1%. Different frequencies were found in wheat cultivars from different zones, which were 0, 3.0%, 21.4%, 16.7%, and 33.3% in North Plain Winter Wheat Region (NPWWR), Yellow-Huai Facultative Winter Wheat Region (YHFWWR), Middle-Lower Yangtze Valley Winter Wheat Region (MLYVWWR), Southwest Winter Wheat Region (SWWR), and Northwest Spring Wheat Region (NSWR), respectively. Whereas, 85.1% of 422 landraces contained Lr34/Yr18, with the frequencies of 89.6%, 77.4%, 93.1%, 93.8%, 96.6%, and 61.1% in NPWWR, YHFWWR, MLYVWWR, SWWR, Southern China Winter Wheat Region (SCWWR) and NSWR, re-spectively. The marker csLV34 exhibited a good repeatability, and can be therefore used for the identification of Lr34/Yr18 in wheat breeding programs. |
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| Keywords: | Common wheat (Triticum aestivum L ) Lr34/Yr18 STS marker csLV34 Marker-assisted selection |
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