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薄皮核桃组织培养与快速繁殖
引用本文:苗玉青,李冠,吴松林,宋祥春,张铁刚.薄皮核桃组织培养与快速繁殖[J].新疆农业科学,2010,47(3):503-507.
作者姓名:苗玉青  李冠  吴松林  宋祥春  张铁刚
作者单位:新疆大学生命科学与技术学院,乌鲁木齐,830046
基金项目:新疆高校科研计划重点项目 
摘    要:目的]研究薄皮核桃组织培养与快速繁殖的方法.方法]以薄皮核桃腋芽为外植体,DKW为基本培养基,通过不同的激素种类和浓度配比,建立薄皮核桃组培快繁体系.结果]组织培养与快速繁殖最适培养基为:启动培养基:DKW/MS+6-BA 1.0 mg/L+IBA 0.01 mg/L;增殖培养基:DKW+6-BA 1.0 mg/L+IBA 0.05 mg/L;壮芽培养基:DKW+6-BA 0.5 mg/L+IBA 0.05 mg/L;生根培养基:1/2 DKW+IBA1.0mg/L,生根率可达70;以上.结论]研究筛选获得适宜薄皮核桃快繁的最佳培养条件,为建立新疆薄皮核桃快繁体系、扩大优质薄皮核桃种植规模奠定一定基础.

关 键 词:薄皮核桃  组织培养  快速繁殖
收稿时间:2010-03-25

Tissue Culture and Rapid Propagation of Thin-shell Walnut
MIAO Yu-qing,LI Guan,WU Song-lin,SONG Xiang-chun,ZHANG Tie-gang.Tissue Culture and Rapid Propagation of Thin-shell Walnut[J].Xinjiang Agricultural Sciences,2010,47(3):503-507.
Authors:MIAO Yu-qing  LI Guan  WU Song-lin  SONG Xiang-chun  ZHANG Tie-gang
Abstract:【Objective】The aim of this text was minly to study the method of the tissue culture and rapid propagation of thin-shell walnut in Xinjiang.【Method】The new axillary shoots segment of thin-shell walnut were used as explants which were cultivated in different DKW media with different types and concentrations of plant hormones.【Result】The optimum culture medium for shoot induction was DKW/MS +6-BA 1.0 mg/L+ IBA 0.01mg/L;The medium for shoot propagation was DKW+ 6-BA 1.0 mg/L+IBA 0.05 mg/L;The suitable medium of the single plantlet growth was DKW+ 6-BA 0.5 mg/L+IBA 0.05 mg/L;The rooting medium was 1/2DKW+ IBA 1.0 mg/L,The rooting rate was up to 70%.【Conclusion】The optimal culture conditions of rapid propagation of thin-shell walnut were selected,which laid the foundation of rapid propagation of thin-shell walnut and amplifying its planting scale.
Keywords:thin-shell walnut  tissue culture  rapid propagation
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