| 兔抗双酚A多克隆抗体的制备 |
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| 引用本文： | 石春红,姚卫蓉,孙秀兰,纪丽君.兔抗双酚A多克隆抗体的制备[J].安徽农业科学,2010,38(8):3886-3888. |
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| 作者姓名： | 石春红 姚卫蓉 孙秀兰 纪丽君 |
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| 作者单位： | 浙江省海通食品集团股份有限公司检测中心,浙江宁波,315300;江南大学食品学院,江苏无锡,214122 |
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| 摘 要： | 目的]通过动物免疫制备抗双酚A多克隆抗体。方法]采用双酚A半抗原（BPAH）和双酚酸（DPA）分别与牛血清蛋白（BSA）偶联制备免疫抗原,免疫新西兰白兔获得抗血清;采用间接竞争ELISA法测定抗血清的效价。结果]经紫外扫描及红外光谱分析,完全抗原BPAH-BSA及DPA-BSA制备成功;供试3只阳性大白兔的抗血清效价分别为〉51200、25600和12800,完全抗原BPAH-BSA免疫的大白兔抗血清效价高于DPA-BSA免疫的大白兔抗血清效价。结论]该研究成功制备了高效价抗双酚A多克隆抗体。
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| 关 键 词： | 双酚A 多克隆抗体 抗血清 效价 |
Preparation of Polyclonal Antibody of Rabbit Anti-bisphenol A |
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| SHI Chun-hong et al.Preparation of Polyclonal Antibody of Rabbit Anti-bisphenol A[J].Journal of Anhui Agricultural Sciences,2010,38(8):3886-3888. |
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| Authors: | SHI Chun-hong |
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| Institution: | Test Center of Haitong Food Group Limited Company of Zhejiang Province;Ningbo;Zhejiang 315300 |
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| Abstract: | Objective] The aim was to prepare polyclonal antibody of anti-bisphenol A through immuning animals.Method] The immune antigen was prepared with bisphenol A hapten (BPAH) and diphenolic acid (DPA) coupled with bovine serum albumin (BSA),and the New zealand white rabbits were immunized with the antigen to prepare antiserum.The antiserum titer was determined by indirect competition ELISA.Result] Through ultraviolet scanning and infrared spectrum analysis,the complete antigen BPAH-BSA and DPA-BSA were succes... |
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| Keywords: | Bisphenol A Polyclonal antibody Antiserum Titer |
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