Triploidy induction by hydrostatic pressure in the pearl oyster, Pinctada martensii Dunker |
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| Authors: | Yi-ping Shen Xi-yuan Zhang Hai-ping He Li-jun Ma |
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| Institution: | Biology Department, Wuhan University, Wuhan, People's Republic of China |
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| Abstract: | Triploidy of the pearl oyster, Pinctada martensii Dunker, was induced with hydrostatic pressure shock. The efficiency of inductions changed with the time of initiation of treatment, the duration of treatment and the intensity of pressure. The most effective procedure for inducing triploidy was the treatment of eggs at 200–250 kg/cm2 5–7 min (for the first polar body retention) and 17–19 min (for the second polar body retention) after insemination for 10 min duration, which resulted in 76% triploid embryos. Under this optimal treatment, high hatchability was also obtained. |
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