| 山羊早孕因子的测定及其特性的研究 |
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| 引用本文： | 陈东宝,张岳.山羊早孕因子的测定及其特性的研究[J].西北农业学报,1992,1(4):75-79. |
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| 作者姓名： | 陈东宝 张岳 |
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| 作者单位： | 湖北省农业科学院畜牧兽医研究所 武昌县 430209;西北农业大学畜牧系 陕西杨陵 712100 |
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| 摘 要： | 怀孕小鼠血清花环抑制滴度显著地高于未孕鼠(p<0.01),说明怀孕血清中存在早孕因子.山羊血清经DEAE—纤维素柱层析、0.05molPBS液(pH7.0)洗脱,收集最初5ml洗脱液并用蔗糖进行浓缩,然后采用改良的小鼠脾细胞花环抑制实验对其花环抑制能力进行测定,结果发现怀孕山羊血清的花环抑制滴度显著地高于未孕山革、发情山革和公革(p<0.001),说明在怀孕山羊血清中也存在早孕因子.进一步研究发现在配种后24h时,山羊血清中即可测出早孕因子活性.而且,怀孕山羊血清早孕因子活性在配种后21天内维持在较高水平,结果提示血清早孕因子可做为山羊早孕诊断的指标.
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| 关 键 词： | 早孕因子 山羊 测定 |
| 收稿时间： | 1992/8/5 0:00:00 |
| 修稿时间： | 1995/9/29 0:00:00 |
A STUDY ON CAPRINE EARLY PREGNANCY FACTOR:DETECTION AND CHARACTERISTS |
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| Chen Dongbao and Zhang Yue.A STUDY ON CAPRINE EARLY PREGNANCY FACTOR:DETECTION AND CHARACTERISTS[J].Acta Agriculturae Boreali-occidentalis Sinica,1992,1(4):75-79. |
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| Authors: | Chen Dongbao and Zhang Yue |
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| Institution: | Institute of Animal Science and Veterinary Medicine, Hubei Agricultural Academy, Wuchang 430209;Northwestern Agricultural University Yangling, Shaanxi 712100 |
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| Abstract: | Aiming at determinipg serum early pregnancy facteor and studing its characteristics in goats, a modified rosette inhibition test witth mouse spleen cells was set up in present study.The rosette inhibition titres of pregnant mouse sera were significantly higher than that of non-pregnant mouse sera (p<0.01),which indicated early pregnancy factor did exsist in pregnant mouse serum.After fractionated with DEAE-cellulose and eluted with pH7.0, 0.05mo1 PBS buffer, the mean rosette inhjbition titre in caprine pregnant sera was significantly higher than that of non-pregnant, oestrus, and male goats (p<0.001),but serum rosette inhibition titres among the goats under late three phsiological status were observed at the same level.These results showed that early pregnancy factor also exsisted in pregnant serum in goats.Moreover, the early pregnancy factor activity apgeared within 29 h after: mating, and sustained at pregnant level untill day 21 after mating.These resurfs impliod that detection of caprine early pregnancy factor in serum could be used in early diagnosis of pregnancy in goats. |
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| Keywords: | Rosette inhibition test Early pregnancy factor Goats |
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