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Variation of leaf esterases in some Ethiopian tetraploid wheat landraces
Authors:Seifu Tsegaya  H C Becker  Tesfaye Tesemma
Institution:(1) Department of Plant Breeding Research, The Swedish University of Agricultural Sciences, S-26831 Svalöv, Sweden;(2) Debre Zeit Agricultural Research Center, Debre Zeit, P.O.Box 32, Ethiopia
Abstract:Summary Esterase isozymes of tetraploid wheat landraces originating from four localities in the central highlands of Ethiopia were studied using the horizontal starch gel electrophoresis. The landraces studied consisted of 60 Triticum turgidum L., one T. dicoccon Schrank, one T. polonicum L. and an improved local cultivar. One introduced durum cultivar was also included for comparison.A total of 12 esterase zymograms were detected in the landraces. Among these, the types C, A and G were found to be the most prevalent. The esterase isozymes exhibited high variation in the landraces. The esterase zymograms of the introduced durum cultivar and T. dicoccon differed from those of the landraces while the patterns attained in T. polonicum and the improved local cultivar were identical to those of the other landraces.The total number of bands detected was 10, of which 2 were rare, being displayed by only 1 pattern each. The band content of zymograms was in the range of 3–6 and the average was 4. The unique bands were observed in zymograms with both high and low prevalences in the landraces. Two zymograms, including zymogram C which had the highest frequency, occurred in all the localities. The high yielding landraces displayed 4 of the zymograms, one of which belonged to two of the zymograms with widest distribution.
Keywords:esterases  Ethiopia  tetraploid wheat landraces  Triticum turgidum  zymograms
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