| 新型伪狂犬病病毒基因缺失株的构建及生物学特性研究(初报) |
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| 引用本文： | 郭万柱,徐志文,王小玉,程陆,王印,汪铭书.新型伪狂犬病病毒基因缺失株的构建及生物学特性研究(初报)[J].四川农业大学学报,2000,18(1):1-3. |
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| 作者姓名： | 郭万柱 徐志文 王小玉 程陆 王印 汪铭书 |
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| 作者单位： | 四川农业大学,动物生物技术中心,四川,雅安,625014 |
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| 基金项目： | 国家自然科学基金! (批准号 :395 70 5 44 )，国家“九五”攻关项目资助。 |
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| 摘 要： | 以伪狂犬病病毒Fa株为起始材料 ,构建的 pp6 3LacZ转移载体质粒与PRVFaDNA经磷酸钙转染 ,在MDBK细胞内同源重组获得PRVFagE-/gI-/LacZ基因缺失株。以PDTK3 - 6 ,5 - 6TK缺失质粒与上述基因缺失株在TK-143细胞内同源重组 ,获得PRV -SA2 15等三基因缺失株。经核酸杂交证实构建是成功的。生物学特性观察显示 ,该基因缺失株对多种动物安全 ,具有良好的免疫原性 ,有望成为一株优秀的PRV疫苗候选株。
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| 关 键 词： | 伪狂犬病病毒 基因缺失 疫苗 生物学特性 |
Construction of new generation Pseudorabies virus gene deleted vaccine and the study of biologic characteristics |
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| GUO Wan-zhu,XU Zhi-wen,Wang Xiao-yu,CHENG Lu,WANG Yin,WANG Ming-shu.Construction of new generation Pseudorabies virus gene deleted vaccine and the study of biologic characteristics[J].Journal of Sichuan Agricultural University,2000,18(1):1-3. |
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| Authors: | GUO Wan-zhu XU Zhi-wen Wang Xiao-yu CHENG Lu WANG Yin WANG Ming-shu |
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| Abstract: | By cotransfection of PRV Fa DNA and the shuttle vector plasmid pp63LacZ DNA in MDBK cells, a deletion mutant strain PRV Fa gE-/gI-/LacZ was constructed with calcium phosphate transfection system. By cotransfection of PRV Fa gE-/gI-/LacZ DNA and TK gene deleted plasmid PDTK3-6, 5-6 DNA, recombinant viruses were then selected for 5'-BUDR resistance in TK-143 cells and Assayed by using the methods of Dot-Blot hybridization, the results suggest PRV Fa TK-/gE-/gI-/LacZ is constructed successfully, the study of biologic characteristics show that vaccination various animals with the gene deleted strain is safe and effective. |
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| Keywords: | PSEUDORABIES VIRUS GENE DELETED VACCINE BIOLOGIC CHARACTERISTICS |
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