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Suppression of damping-off of cucumber caused by Pythium ultimum with live cells and extracts of Serratia marcescens N4-5 |
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| Authors: | DP Roberts LF McKenna SLF Meyer JT de Souza J Lydon JS Buyer |
| Institution: | a Sustainable Agricultural Systems Laboratory, USDA—Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705, USA b Floral and Nursery Plants Unit, USDA—Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705, USA c Nematology Laboratory, USDA—Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705, USA d University of Maryland, Wye Research and Education Center, Queenstown, MD 21658, USA e Molecular Plant Pathology Laboratory, USDA—Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705, USA f Department of Applied Microbiology, Yeungnam University, Gyeongsan, 712-749 Gyeongbuk, Republic of Korea |
| Abstract: | Environmentally friendly control measures are needed for the soil-borne pathogen, Pythium ultimum. This pathogen can cause severe losses to field- and greenhouse-grown cucumber and other cucurbits. Live cells and ethanol extracts of cultures of the bacterium Serratia marcescens N4-5 provided significant suppression of damping-off of cucumber caused by P. ultimum when applied as a seed treatment. Live cells of this bacterium also suppressed damping-off caused by P. ultimum on cantaloupe, muskmelon, and pumpkin. Culture filtrates from strain N4-5 contained chitinase and protease activities while ethanol extracts contained the antibiotic prodigiosin, the surfactant serrawettin W1, and possibly other unidentified surfactants. Production of prodigiosin and serrawettin W1 was temperature-dependent, both compounds being detected in extracts from N4-5 grown at 28 °C but not in extracts from N4-5 grown at 37 °C. Ethanol extracts from strain N4-5 grown at 28 °C inhibited germination of sporangia and mycelial growth by P. ultimum in in vitro experiments. There was no in vitro inhibition of P. ultimum associated with ethanol extracts of strain N4-5 grown at 37 °C. Prodigiosin, purified from two consecutive thin-layer chromatography runs using different solvent systems, inhibited germination of sporangia and mycelial growth of P. ultimum. Another unidentified compound(s) also inhibited germination of sporangia but did not inhibit mycelial growth. There was no in vitro inhibition associated with serrawettin W1. These results demonstrate that live cells and cell-free extracts of S. marcescens N4-5 are effective for suppression of damping-off of cucumber caused by P. ultimum possibly due in part to the production of the antibiotic prodigiosin. |
| Keywords: | Biological control Cucumber Damping-off Meloidogyne incognita Prodigiosin Pythium ultimum Seed treatments Serratia marcescens |
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