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| 牛瘦素受体基因表达及其结合瘦素特性研究 | |
| 引用本文： | 李林强,田万强,昝林森.牛瘦素受体基因表达及其结合瘦素特性研究[J].中国牛业科学,2011,37(4):1-5. |
| 作者姓名： | 李林强 田万强 昝林森 |
| 作者单位： | 1. 陕西师范大学食品工程与营养科学学院,陕西 西安,710062 2. 杨凌职业技术学院动物工程系,陕西 杨凌 712100;西北农林科技大学动物科技学院,陕西 杨凌 712100 3. 西北农林科技大学动物科技学院,陕两 杨凌,712100 |
| 基金项目： | 陕西省13115科技创新工程，教育部长江学者与创新团队发展支持计划 |
| 摘 要： | 目的]以秦川牛为研究对象,克隆、原核表达秦川牛瘦素受体(Lepr)基因的蛋白功能区,分析表达蛋白对瘦素结合特性,为研究瘦素受体与瘦素结合的调节机制提供理论基础.方法]本研究通过RT-PCR法从秦川牛肉mRNA扩增获得Lepr IG基因的cDNA序列,克隆至pMD18-T载体获得重组质粒,并进行序列测定.将测序正确的... |
| 关 键 词： | 牛 瘦素受体 IG基因 克隆 表达 |
Studies on Protein Expression of Functional Region of Leptin Receptor Gene and its Character of Combinding Leptin in Cattle |
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| LI Lin-qiang,TIAN Wan-qiang,ZAN Lin-sen.Studies on Protein Expression of Functional Region of Leptin Receptor Gene and its Character of Combinding Leptin in Cattle[J].China Cattle Science,2011,37(4):1-5. | |
| Authors: | LI Lin-qiang TIAN Wan-qiang ZAN Lin-sen |
| Institution: | 1.College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an,Shaanxi 710062,China;2.Department of Animal Engineering,Yangling Vocational & Technical College,Y angling,Shaanxi 712100,China;3 College of Animal Science and Technology,Northwest A&F Universit,Yangling,Shaanxi 712100,China) |
| Abstract: | 【Objective】In this paper,the functional region of leptin receptor IG gene was cloned and expressed,the expressed protein combining leptin properties were analyzed,which would provide theoretical basis for the regulation of Lepr and leptin combinding.【Method】 The cDNA of Lepr IG gene was amplified from muscle mRNA of Qinchuan cattle by RT-PCR.The PCR product was cloned into the T vector pMD18-T to construct plasmid for sequencing.Then the cDNA was subcloned into the prokaryotic expressing plasmid vector pET30a(NdeI/Xho1) and transformed into host Escherichia coli strain BL2l(DE3) for expression.The expression of Lepr function protein was induced by IPTG,and was identified by SDS-PAGE.The protein was purified by Ni NTA column.Combinding leptin function of the expressed recombinant protein via high performance liquid chromatography frontal analysis(HPLC-FA).【Result】The results showed that Lepr IG gene was highly expressed in Escherichia coli,and the expression product was observed with soluble protein and inclusion body,the expressed recombinant protein had the function of combinding leptin.【Conclusion】In vitro expressed exogenous,Lepr IG gene protein had combinding leptin function and the study provided the foundation for inhibitting Qinchuan cattle energy metabolism and fat deposition through regulation of Lepr on combinding leptin. |
| Keywords: | Cattle leptin Receptor IG gene Cloning Expression |
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