| 缢蛏六群体16S rRNA基因片段序列的差异分析 |
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| 引用本文： | 牛东红,李家乐,汪桂玲,姜志勇,张文博,沈玉帮,冯冰冰.缢蛏六群体16S rRNA基因片段序列的差异分析[J].上海水产大学学报,2007,16(1):1-6. |
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| 作者姓名： | 牛东红 李家乐 汪桂玲 姜志勇 张文博 沈玉帮 冯冰冰 |
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| 作者单位： | 上海水产大学生命科学与技术学院,上海200090 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 采用PCR技术扩增了缢蛏线粒体DNA的16SrRNA基因片段，PCR产物经纯化、测序、同源序列比对获得长度为440bp的核苷酸序列。利用16SrRNA基因片段分析了江浙沪地区三个野生群体（江苏射阳、上海崇明、浙江象山）和三个养殖群体（江苏射阳、上海奉贤、浙江象山）的遗传多样性，共检测到了19个单倍型和41个核苷酸多态位点。序列分析结果显示，三个野生群体之间出现了明显的遗传分化，其中崇明群体遗传多样性最高，其次为射阳群体，象山群体遗传多样性最低，表明崇明群体未受到养殖群体基因的污染。在养殖群体之间则没有达到遗传分化，且单倍型混杂，聚类结果显示与象山野生群体亲缘关系最近，这表明长期的养殖过程在一定程度上对野生群体产生了影响，降低了种质资源的丰富度。
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| 关 键 词： | 缢蛏 线粒体DNA 遗传多样性 |
| 文章编号： | 1004-7271（2007）01-0001-06 |
| 收稿时间： | 2006-10-08 |
| 修稿时间： | 2006年10月8日 |
The genetic diversity of mitochondrial 16S rRNA gene fragment in six populations of Sinonovacula constricta |
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| NIU Dong-hong,LI Jia-le,WANG Gui-ling,JIANG Zhi-yong,ZHANG Wen-bo,SHEN Yu-bang,FENG Bing-bing.The genetic diversity of mitochondrial 16S rRNA gene fragment in six populations of Sinonovacula constricta[J].Journal of Shanghai Fisheries University,2007,16(1):1-6. |
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| Authors: | NIU Dong-hong LI Jia-le WANG Gui-ling JIANG Zhi-yong ZHANG Wen-bo SHEN Yu-bang FENG Bing-bing |
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| Abstract: | Mitochondrial 16S rRNA gene fragment of Sinonovacula constricta was amplified with polymerase chain reaction (PCR). The PCR products were purified and sequenced from three wild populations: She-yang (WS), Chong-ming (WC), Xiang-shan (WX) and three cultured populations She-yang (CS), Feng-xian (CF), Xiang-Shan (CX) in Jiangsu, Zhejiang and Shanghai. 440 base-pair nucleotide sequences of 16 SrRNA were examined and analyzed for genetic polymorphism. Sequences data analysis showed that all 47 sequences were grouped into 19 haplotypes and there were 41 variable nucleotide positions in 16S rRNA gene fragments. The genetic diversity indexes were calculated and the results indicate that the significant genetic difference was observed among three wild populations, WC population has more variation than others, followed by WS population, WX population has the least sequence variation,which suggests that WC population has not been polluted by cultured populations. But there is no genetic difference among three cultured populations and it is closest to WX population. So the culturing process has reduced wild genetic diversity. |
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| Keywords: | 16S rRNA |
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