Construction of Antibacterial Peptide CecropinB Eukaryotic Recombinant Vector and Its Expression in Dairy Goat Mammary Gland Epithelial Cells |
| |
| Authors: | GAO Xuejun TONG Huili YIN Deyun ZHANG Li Key Laboratory of Dairy Science Ministry of Education Northeast Agricultural University Harbin China |
| |
| Institution: | Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China |
| |
| Abstract: | To investigate the expression of antibacterial peptide CecropinB eDNA in dairy goat mammary gland epithelial cells, the CecropinB gene was eloned and was inserted into a eukaryotic vector pECFP-Cl to construct the recombinant plasmid pECFP-B by genetic engineering technique. Recombinant plasmid pECFP-B was transfected into dairy goat mammary gland epithelial to detect the bactericidal activity of CeeropinB. The expression of CecropinB was also detected. The result of RT-PCR demonstrated CecropinB gene was expressed in transfeeted cells. CecropinB recombinant plasmid DNA was injected into udders and CecropinB was expressed in mammary gland, exhibiting bactericidal activity to Staphylococcus aureus in vivo experiments. |
| |
| Keywords: | CecropinB goat mammary epithelial gland cell transfection bactericidal activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
