| 基于Special-Base模型检测转基因作物nos/plamid构建特异性 |
| |
| 引用本文： | 兰青阔,王永,赵新,陈锐,朱珠,李欧静,余景会,郭永泽,程奕.基于Special-Base模型检测转基因作物nos/plamid构建特异性[J].中国农业大学学报,2012,17(2):8-12. |
| |
| 作者姓名： | 兰青阔 王永 赵新 陈锐 朱珠 李欧静 余景会 郭永泽 程奕 |
| |
| 作者单位： | 天津市农业科学院中心实验室／农业部转基因产品成分监督检验测试中心,天津,300381 |
| |
| 基金项目： | 科技部国际合作项目(2006DFA32380) |
| |
| 摘 要： | 以转基因作物中常见的nos/plamid构建特异性序列为研究对象,通过同源性比较和焦磷酸测序dispensation order设计,建立Special-Base焦磷酸测序检测模型;利用转基因大豆Roundup Ready Soybean和转基因玉米Bt11、GA21、NK603等4种转基因作物为试验材料,针对nos/plamid构建特异性序列设计引物,经复合PCR扩增及焦磷酸测序,验证Special-Base焦磷酸测序检测模型有效性。结果表明:本研究建立的Special-Base焦磷酸测序模型能检测出样品中单一或多种转基因作物成分,提高了转基因作物检测的精确性、检测通量和自动化程度。
|
| 关 键 词： | 转基因作物(GMC) 复合PCR 焦磷酸测序 Special-Base nos/plamid |
| 收稿时间： | 2011/11/28 0:00:00 |
Special-Base GMCs detection using multiple PCR and pyrosequencing |
| |
| LAN Qing-kuo,WANG Yong,ZHAO Xin,CHEN Rui,ZHU Zhu,LI Ou-jing,YU Jing-hui,GUO Yong-ze and CHENG Yi.Special-Base GMCs detection using multiple PCR and pyrosequencing[J].Journal of China Agricultural University,2012,17(2):8-12. |
| |
| Authors: | LAN Qing-kuo WANG Yong ZHAO Xin CHEN Rui ZHU Zhu LI Ou-jing YU Jing-hui GUO Yong-ze and CHENG Yi |
| |
| Institution: | (Central Lab/Supervision and Test Center for Molecular Characteristics of Genetically Modified Organisms and Their Derivates of Ministry of Agriculture,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China) |
| |
| Abstract: | Despite the various technologies in place for GMC test,a robostic method is highly demanded.The technology described here represents a breakthrough development in that direction.Aimming to nos/plamid gene of four GMCs(RRS,Bt11,GA21,NK603),by combining the multiple PCR with pyrosequencing,we developed a rapid assay for genotyping nos/plamid that relies on the identification of a single,type-specific ’sentinel’ base.The prototype assay was developed to recognize the four GMCs and was capable of recognizing simultaneously multiple GMCs infections.The studies established a rapid,reliable and cost-effective assay. |
| |
| Keywords: | GMCs multiple PCR pyrosequencing Special-Base nos/plamid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
|
