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| 用正交试验法优选绿盲蝽若虫人工饲料配方 | |
| 引用本文： | 宋国晶,李国平,封洪强,李海平,邱峰.用正交试验法优选绿盲蝽若虫人工饲料配方[J].植物保护,2010,36(6):96-99. |
| 作者姓名： | 宋国晶 李国平 封洪强 李海平 邱峰 |
| 作者单位： | 1. 河南省农业科学院植物保护研究所,郑州,450002;内蒙古农业大学农学院,呼和浩特,010019 2. 河南省农业科学院植物保护研究所,郑州,450002 3. 内蒙古农业大学农学院,呼和浩特,010019 |
| 基金项目： | 公益性行业科研专项经费项目，河南省财政厅项目 |
| 摘 要： | 采用10因子3水平正交试验,得出影响绿盲蝽若虫总历期的人工饲料各因子顺序为:鸡蛋啤酒酵母小麦胚大豆卵磷脂、复合维生素卵黄利马豆粉蜂蜜水蔗糖大豆粉;影响绿盲蝽若虫存活率的因子顺序为:鸡蛋啤酒酵母利马豆粉大豆卵磷脂、复合维生素大豆粉蔗糖蜂蜜水小麦胚卵黄。综合两种结果,优选出绿盲蝽若虫人工饲料配方,其成分如下:蔗糖2.8 g、啤酒酵母粉0.25 g、50%蜂蜜水2.5 g、鸡蛋22.5 g、小麦胚10 g、利马豆粉30 g、大豆粉2.5 g、卵黄30 g、大豆卵磷脂1.5 g、复合维生素1.2 g、水164 g。此优化配方饲养绿盲蝽若虫存活率为80.57%,与四季豆饲养的若虫存活率71.13%没有显著差异;若虫总历期为11.80 d,较四季豆饲养的总历期10.4 d延长约1 d。 |
| 关 键 词： | 绿盲蝽 若虫 人工饲料 正交试验 关键因子 |
Optimization of artificial diet composition for Apolygus lucorum nymphs by orthogonal design |
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| Song Guojing,Li Guoping,Feng Hongqiang,Li Haiping,Qiu Feng.Optimization of artificial diet composition for Apolygus lucorum nymphs by orthogonal design[J].Plant Protection,2010,36(6):96-99. | |
| Authors: | Song Guojing Li Guoping Feng Hongqiang Li Haiping Qiu Feng |
| Institution: | 1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou450002, China;2. College of Agronomy, Inner Mongolia Agricultural University, Hohhot010019, China |
| Abstract: | The composition of artificial diet for Apolygus lucorum Meyer Dür nymphs was optimized by orthogonal design trials with 10 factors and 3 levels. The factors affecting the developmental duration of nymphs were as followed in that order: chicken eggs, brewer’s yeast, wheat germ, soy lecithin, Vanderzant vitamin mixture, egg yolk, lima bean meal, 50% honey solution, sucrose and soy flour, and the factors affecting nymphal survival were as followed in that order: chicken eggs, brewer’s yeast, lima bean meal, soy lecithin, Vanderzant vitamin mixture, soy flour, sucrose, 50% honey solution, wheat germ and egg yolk. Based on the above results, the optimized artificial diet composition for A. lucorum nymphs was sucrose 2.8 g, brewer’s yeast 0.25 g, 50% honey solution 2.5 g, chicken eggs 22.5 g, wheat germ 10 g, lima bean meal 30 g, soy flour 2.5 g, egg yolk 30 g, soy lecithin 1.5 g, Vanderzant vitamin mixture 1.2 g, and water 164 g. There were no significant differences in nymphal survival between the optimized artificial diet and green beans. However, the duration of nymphs fed with the optimized artificial diet was lengthened compared with those on green beans. |
| Keywords: | Apolygus lucorum nymph artificial diet orthogonal design key factor |
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