| 日本木立芦荟离体快繁体系研究 |
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| 引用本文： | 徐重益,傅术琳,程智慧.日本木立芦荟离体快繁体系研究[J].西北农林科技大学学报(社会科学版),2004,32(6):83-86. |
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| 作者姓名： | 徐重益 傅术琳 程智慧 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨凌,712100 |
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| 摘 要： | 以日本木立芦荟(A.arborescensMill.)茎尖和幼嫩茎段为外植体进行离体快繁。结果表明,茎尖在MS+6-BA3.0mg/L+NAA0.2mg/L培养基上形成的不定芽数最多,达21个;而茎段在MS+6-BA4.0mg/L+NAA0.1mg/L培养基上诱导的不定芽数最多,为8.1个。MS+6-BA3.0mg/L+B9100mg/L培养基对不定芽的增殖效果最好,平均每个芽可增殖不定芽11.5个。1.0mg/LNAA和2.0mg/LIBA能显著促进不定芽生根,生长延缓剂CCC也具有促进生根作用。将组培苗移栽到体积比组成为2份有机厩肥+1份园田土或2份蛭石+1份园田土的基质中,成活率可达90%以上。
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| 关 键 词： | 木立芦荟 离体快繁 器官发生 组织培养 |
| 文章编号： | 1671-9387(2004)06-0083-04 |
| 收稿时间： | 2003/12/22 0:00:00 |
| 修稿时间： | 2003年12月22 |
Study on tissue culture of A.arborescens Mill |
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| XU Chong-yi,FU Shu-lin,CHENG Zhi-hui.Study on tissue culture of A.arborescens Mill[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(6):83-86. |
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| Authors: | XU Chong-yi FU Shu-lin CHENG Zhi-hui |
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| Institution: | (College of Horticulture,Norwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | Tissue culture of A.arborescens was studied with meristem and young stem segments as explants.The optimum media for induction of adventitious buds from meristem and stem segment were MS+6-BA 3.0 mg/L+NAA 0.2 mg/L and MS+6-BA 4.0 mg/L+NAA 0.1 mg/L respectively with the bud number reaching to 21 and 8.1.Shoots were propagated as much as 11.5 when they were placed at MS+6-BA 3.0 mg/L+B9(duminozide) 100 mg/L.1.0 mg/L NAA and 2.0 mg/L IBA were the best treatments for the induction of roots.Besides NAA and IBA,CCC a kind of inhibitor for growth also was helpful to promote shoot to root differentiation.More than 90% of the shoots with roots survived after planted into mixture of 2 stable manure+1 soil or 2 vermiculite+1 soil. |
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| Keywords: | A arborescens Mill rapid propagation in vitro organogeny tissue culture |
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