| 结球甘蓝的组织培养与快繁研究 |
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| 引用本文： | 石晓云.结球甘蓝的组织培养与快繁研究[J].安徽农业科学,2010,38(32):18077-18078. |
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| 作者姓名： | 石晓云 |
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| 作者单位： | 河北省邢台学院生物·化学系,河北邢台,054001 |
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| 摘 要： | 目的]研究适合结球甘蓝组织培养与快速繁殖的最佳方法与培养基种类。方法]以结球甘蓝无菌苗的胚轴和子叶为材料,以MS为诱导和增殖的基本培养基,以1/2MS为生根基本培养基,比较不同浓度及配比的生长调节物质对增殖及防止玻璃化苗的影响。结果]接种于诱导培养基MS＋6-BA 3.00 mg/L＋NAA 0.10 mg/L中的子叶可长出愈伤组织,胚轴可直接分化不定芽;在增殖培养基MS＋6-BA 1.00 mg/L＋NAA 0.10 mg/L上生长的组培苗生长势强,玻璃化苗数最少,增殖率最高,平均增殖系数为3.8;接种于生根培养基1/2MS＋NAA 0.10 mg/L和1/2MS＋IBA 0.20 mg/L上的组培苗生根率最高,达100%。结论]最佳诱导培养基为MS＋6-BA3.00 mg/L＋NAA 0.10 mg/L;最佳增殖培养基为MS＋6-BA 1.00 mg/L＋NAA 0.10 mg/L;最佳生根培养基为1/2MS＋NAA 0.10 mg/L和1/2MS＋IBA 0.20 mg/L。
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| 关 键 词： | 结球甘蓝 组织培养 快繁 玻璃化 |
Study on Tissue Culture and Rapid Propagation of Brassica oleracea L. var. captitata |
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| SHI Xiao-yun.Study on Tissue Culture and Rapid Propagation of Brassica oleracea L. var. captitata[J].Journal of Anhui Agricultural Sciences,2010,38(32):18077-18078. |
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| Authors: | SHI Xiao-yun |
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| Institution: | SHI Xiao-yun (Department of Biology , Chemistry,College of Xingtai,Xingtai,Hebei 054001) |
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| Abstract: | Objective] To study the optimum way and medium of Brassica oleracea L.var.captitata tissue culture and rapid propagation.Method] The hypocotyls segments and cotyledons of aseptic seedlings of Brassica oleracea L.var.captitata were used as explants.MS was the basic induction and propagation medium.1/2MS was the basic rooting medium.The effect of different concentration and ratio on propagation and confining the vitreous seedlings were compared.Result]The cotyledons inoculated to induction medium MS+6-BA 3... |
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| Keywords: | Brassica oleracea L var capitata Tissue Culture Rapid Propagation Vitreous |
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