| 芽孢杆菌对草鱼生长和肠粘膜抗氧化功能及养殖水质的影响 |
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| 引用本文： | 李卫芬,邓斌,陈南南,梁权,周绪霞.芽孢杆菌对草鱼生长和肠粘膜抗氧化功能及养殖水质的影响[J].水生态学杂志,2012,33(1):65-70. |
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| 作者姓名： | 李卫芬 邓斌 陈南南 梁权 周绪霞 |
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| 作者单位： | 1. 浙江大学动物科学学院教育部动物分子营养重点实验室,杭州,310029
2. 浙江工业大学生物与环境工程学院,杭州,310014 |
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| 基金项目： | 国家重点基础研究发展计划（973计划） |
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| 摘 要： | 本试验研究了饲料中添加芽孢杆菌对草鱼生长、肠粘膜抗氧化功能及养殖水体水质的影响。选取平均体重为（51.0±2.3）g的健康草鱼300尾，随机分成3组（对照组、处理组1和处理组2），每组3个重复，每个重复50尾鱼。其中对照组饲喂基础日粮，处理组1和2分别饲喂含复合芽孢杆菌（105 cfu/克饲料，枯草芽孢杆菌和解淀粉芽孢杆菌以1:1比例混合）和枯草芽孢杆菌（105 cfu/克饲料）的基础日粮。试验期为45d。结果表明，饲料中添加芽孢杆菌对养殖水体pH和硝酸盐氮含量无显著影响，但显著降低了从第21d到试验结束期间水体中亚硝酸盐氮的含量（处理组1第35d除外）。芽孢杆菌的添加同时显著降低草鱼的死亡率（P<0.05），并提高了草鱼的增重率和特定生长率。与对照组相比，处理组1和2草鱼的增重率分别提高了52.93%（P<0.01）和21.78%（P<0.05），特定生长率分别提高了44.44%（P<0.01）和16.67%（P<0.05）；而且处理组1草鱼增重率和特定生长率分别比处理组2提高了25.58%（P<0.01）和23.81%（P<0.01）。肠粘膜抗氧化活性研究表明，与对照组相比，饲料中添加芽孢杆菌能提高草鱼肠粘膜超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。以上结果提示，饲料中添加芽孢杆菌可以改善养殖水质和草鱼肠粘膜抗氧化功能，并显著促进草鱼生长并降低死亡率。
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| 关 键 词： | 芽孢杆菌 草鱼 水质 生长性能 抗氧化功能 |
| 收稿时间： | 2010/11/13 0:00:00 |
| 修稿时间： | 2011/12/12 0:00:00 |
Effects of Bacillus on Water Quality, Growth Performance and Antioxidant Activity of Intestinal Mucosa of Grass Carp |
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| LI Wei fen,DENG Bin,CHEN nan nan,LIANG Quan and zhou xu xia.Effects of Bacillus on Water Quality, Growth Performance and Antioxidant Activity of Intestinal Mucosa of Grass Carp[J].Journal of Hydroecology,2012,33(1):65-70. |
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| Authors: | LI Wei fen DENG Bin CHEN nan nan LIANG Quan and zhou xu xia |
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| Institution: | College of Animal Sciences, Zhejiang University,College of Animal Sciences, Zhejiang University,College of Animal Sciences, Zhejiang University,College of Animal Sciences, Zhejiang University,College of Animal Sciences, Zhejiang University |
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| Abstract: | A 45d culture experiment was conducted to study the effect of Bacillus on growth performance, antioxidant activity of intestinal mucosa of grass carp (Ctenopharyngodon idellus) and water quality. 450 grass carps with initial weight of (51.0±2.3) g were randomly divided into 3 groups (three replicates for each group and 50 fish per replicate). Control group was fed the basal diet, Group 1 were fed the basal diet supplemented with Bacillus complex (B. subtilis and B. amyloliquefaciens, 1:1; 105cfu/g diet), and Group 2 were fed the basal diet supplemented with B. subtilis only (105cfu/g diet). The results indicated that compared with the control group, water nitrite nitrogen level in Group 1 and Group 2 were significantly decreased from the 21st day to the end of the experiment (except on the 35th day for group 1, P<0.01); and ammonium nitrogen level in group 2 was also significantly decreased on the 21st and 35th day. No significant differences were found for water pH and nitrate nitrogen level among the three groups. Supplementation of Bacillus also decreased the death rate (P<0.05) and improved the growth performance of grass carps. Compared with the control group, WGR of Group 1 and Group 2 were increased by 52.93% (P<0.01) and 21.78% (P<0.05), and ADFI were increased by 44.44% (P<0.01) and 16.67% (P<0.05), respectively; and compared with Group 2, supplementation of complex Bacillus increased both WGR and ADFI of grass carp significantly (25.58% and 23.81%, respectively, P<0.01). Further study showed that supplementation of Bacillus improved partial antioxidant capacity of intestinal mucosa of grass carp, as evidenced by increased activities of superoxide dismutase (SOD) and catalase (CAT). It can be concluded that, Bacillus supplementation in basal diets can improve water quality and antioxidant capacity of grass carp, and further improve the growth performance and decrease the death rate of grass carp. |
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| Keywords: | Bacillus Grass carp Water quality Growth performance Antioxidant capacity |
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