Nutritional regulation of somatostatin expression in rainbow trout, Oncorhynchus mykiss |
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| Authors: | Melissa M Ehrman Gregory T Melroe Craig A Moore Jeffrey D Kittilson Mark A Sheridan |
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| Institution: | (1) Present address: School of Medicine, University of Minnesota, Minneapolis, MN 55455, USA;(2) Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA |
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| Abstract: | Previously we characterized three DNAs from the endocrine pancreas (Brockmann body) of rainbow trout (Oncorhynchus mykiss) that encode for distinct preprosomatostatin (PPSS) molecules: one containing somatostatin-14 and its C-terminus (PPSS I)
and two containing Tyr7, Gly10]-somatostatin-14 at their C-termini (PPSS II′ and PPSS II″). In the present study, the regulation of PPSS expression was
studied in rainbow trout placed on varying nutritional regimes (fed continuously, fasted, fasted then refed). Fish that were
fasted for one week displayed reduced growth compared to their fed counterparts, but no alteration in pancreatic PPSS expression
was noted between the two groups. Fish fasted for 4 and 6 weeks also were growth retarded and displayed increased levels of
PPSS I mRNA and PPSS II″ mRNA compared to fed animals; PPSS II′ mRNA levels were not affected by food deprivation. Refeeding
fish for two weeks following 4 weeks of food deprivation restored growth and reduced PPSS I and PPSS II″ mRNA expression to
levels similar to those displayed by continuously fed fish. Changes in PPSS expression were correlated with increases in plasma
SS. These results indicate that nutritional state modulates differential expression of PPSSs.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | fasting rainbow trout somatostatin expression |
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