| 亚硝酸氮胁迫下噬菌体防治凡纳滨对虾弧菌病 |
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| 引用本文： | 周鲜娇,邱德全.亚硝酸氮胁迫下噬菌体防治凡纳滨对虾弧菌病[J].水产科学,2011,30(7):378-382. |
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| 作者姓名： | 周鲜娇 邱德全 |
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| 作者单位： | 1. 湛江师范学院生命科学与技术学院,广东,湛江,524048
2. 广东海洋大学水产学院,广东,湛江,524025 |
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| 基金项目： | 广东省自然科学基金资助项目，湛江市科技招标项目 |
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| 摘 要： | 在养殖水体不同亚硝酸氮的条件下,通过对凡纳滨对虾的毒性试验和检测对虾部分免疫指标的变化来研究副溶血弧菌噬菌体对对虾弧菌病的防治效果.试验设置0.005(对照)、0.75、1.50 mg/L和3.00 mg/L 4个不同亚硝酸氮的质量浓度作为胁迫组,并在此基础上加入副溶血弧菌设置为胁迫感染组,加入副溶血弧菌和噬菌体设置为...
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| 关 键 词： | 亚硝酸氮 副溶血弧菌噬菌体 弧菌病 凡纳滨对虾 |
Biological Control of Vibriosis in Pacific White Leg Shrimp Litopenaeus vannamei Under Stresses of Nitrite Nitrogen by Bacteriophages |
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| ZHOU Xian-jiao,QIU De-quan.Biological Control of Vibriosis in Pacific White Leg Shrimp Litopenaeus vannamei Under Stresses of Nitrite Nitrogen by Bacteriophages[J].Fisheries Science,2011,30(7):378-382. |
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| Authors: | ZHOU Xian-jiao QIU De-quan |
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| Institution: | 1.Life Science and Technology Science School,Zhanjiang Normal University,Zhanjiang 524048,China;2.Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | The effect of Vibrio parahaemolyticus bacteriophage on vibriosis and the immunity was studied in Pacific white leg shrimp Litopenaeus vannamei by a toxicity experiment and under different nitrite nitrogen(0.005 mg/L,0.75 mg/L,1.50 mg/L and 3.00 mg/L).The V.parahaemolyticus was added into the nitrite nitrogen stress experiment groups,Thich were named the vibro infection experiment groups.The V.parahaemolyticus bacteriophage was added into the inffection experiment groups which were regarded as the bacteriophage control experiment groups.In the toxicity experiment,the shrimp had no significant difference in mortality between the bacteriophage control groups and the nitrite nitrogen stress groups(P>0.05),but it has significant difference between the bacteriophage experiment control groups and the vibrio infection experiment groups(P<0.05).The total hemocyte count among the three groups were aranged descedantly as the vibro infection roups,the bacteriophage control experiment groups,the nitrite nitrogen stress groups.All the activities of blood serum PO,SOD and ACP increased,and then decreased in all these test groups except the control group(0.005 mg/L);in the 0.005 mg/L,and 0.75 mg/L nitrite nitrogen groups,the activities of blood serum PO,SOD and ACP in the bacteriophage control groups were more than those in the vibrio infection groups(P<0.05),which were less than those in the nitrite nitrogen stress groups(P<0.05),on the 10th day,In the 1.50 mg/L and 3.00 mg/L nitrite nitrogen groups,the activities of blood serum SOD,and ACP in the bacteriophage control groups were more than those in the vibrio infection of(P<0.05),which were less than the nitrogen stress groups(P<0.05).All the results showed that the bacteriophage controled vibrosis effectly only under low concentration of nitrite nitrogen water;under the stress of high nitrite nitrogen,the bacteriophage could relieve the attact of the vibro to the shrimp. |
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| Keywords: | nitrite nitrogen V parahaemolyticus bacteriophage vibriosis Litopenaeus vannamei |
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