High level expression of biologically active canine interferon-alpha subtype 4 using a baculovirus |
| |
| Authors: | Ruttanapumma Ruttapong Anzai Manami Takegawa Masaoki Okamura Masashi Maehara Nobutoshi Sato Ko Yoshioka Kuniaki Itoh Akira Nakamura Masayuki Takehara Kazuaki |
| |
| Institution: | Laboratory of Zoonoses, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. |
| |
| Abstract: | In this study, a high amount of bioactive recombinant canine interferon-alpha subtype 4 (CaIFN-alpha4) was expressed in a baculovirus system. For easy purification, it was expressed as a CaIFN-alpha4 bearing histidine hexamer at the C-terminal region, designated CaIFN-alpha4His. CaIFN-alpha4His was detected in culture supernatants of insect cells infected with the recombinant virus using sodium dodecyl sulfate-polyarcylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining. The level of expression was very high, and approximately 1 mg of purified protein, with 5.0 x 10(7) units/mg, was obtained from 300 ml of culture supernatant. The purified product showed antiviral activity against Vesicular stomatitis virus on canine tumor cell line A72 and chicken embryo fibroblast cells. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
