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| 东北春玉米骨干自交系组织培养再生系统研究 | |
| 引用本文： | 袁鹰; 王玉民; 郝文媛; 谭化;刘德璞,曲喜云,张光弟.东北春玉米骨干自交系组织培养再生系统研究[J].玉米科学,2005,13(3):028-031. |
| 作者姓名： | 袁鹰; 王玉民; 郝文媛; 谭化;刘德璞 曲喜云 张光弟 |
| 作者单位： | 1. 吉林省农业科学院生物技术重点实验室,吉林,公主岭,136100 2. 长春市种子管理站 3. 吉林省梨树县农业技术推广站 |
| 基金项目： | 国家转基因植物中试及产业化基地(吉林)专项资助(JY03-17-1) |
| 摘 要： | 东北玉米骨干自交系在诱导愈伤组织和植株再生中,基因型差异非常显著。自交系7922最高愈伤诱导率达89.1%,芽苗率为66.4%;而最低的921诱导率仅有18.9%,芽苗率为0。再生途径主要有两种:7922主要以体胚发生途径为主,4122、8902主要以器官发生途径为主。2熏4-D是体胚诱导不可缺少的植物生长激素,适当的细胞分裂素能促进诱导4112、8902不定芽的产生。而且,再生途径并不是一成不变的,在一定条件下可以相互转变。 |
| 关 键 词： | 玉米 自交系 组织培养 再生系统 |
| 文章编号： | 1005-0906（2005）03-0028-04 |
| 收稿时间： | 8/3/2004 12:00:00 AM |
| 修稿时间： | 2004年8月3日 |
Study on Regeneration of Spring Maize Inbred Lines in Northeast of China |
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| YUAN Ying; WANG Yu-min,QU Xi-yun.Study on Regeneration of Spring Maize Inbred Lines in Northeast of China[J].Journal of Maize Sciences,2005,13(3):028-031. | |
| Authors: | YUAN Ying; WANG Yu-min QU Xi-yun |
| Abstract: | There existed great differences among maize genotypes when immature embryos were cultured for plant regeneration.7922 showed the highest frequencies of callus induction and shoot formation which were 89.1%,and 66.4% respectively.While the lowest was found in 921,the induction and regeneration frequencies were 18.9% and 0 respectively.There are two regeneration pathways.7922 tended to regenerate via somatic embryogenesis,4112,8902 are likely to regenerate via organogenesis.2,4-D was essential for inducing somatic embryoids,and suitable cytokin can promote adventitious shoot formation of 4112 and 8902.Furthermore,the regeneration pathways are interchangeable under different culture conditions. |
| Keywords: | Maize Inbred Tissue culture Regeneration |
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