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Regulation of gonadotropin subunit genes expression by 11-ketotestosterone during early spermatogenesis in male red seabream, Pagrus major |
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| Authors: | S Yamaguchi K Gen K Okuzawa N Kumakura H Kagawa M Matsuyama |
| Institution: | 1. Department of Animal and Marine Bioresource Sciences, Kyushu University, Fukuoka, 812-8581, Japan 2. Fisheries Research Agency, Inland Station, National Research Institute of Aquaculture, Tamaki, Mie, 519-0423, Japan 3. Fisheries Research Agency, National Research Institute of Aquaculture, Nansei, Mie, 516-0193, Japan 4. Department of Aquatic Bioscience, Tokyo University of Fisheries, Minato, Tokyo, 108-8477, Japan 5. Department of Biological Production and Environmental Science, Miyazaki University, Miyazaki, 889-2192, Japan |
| Abstract: | To examine the roles of gonadal steroids in the regulation of expression of gonadotropin subunit genes, male red seabream were gonadectomized and a sub-group was treated with 11-ketotestosterone (11-KT). Castration of males during the early stage of spermatogenesis elicited a significant increase in FSHβ mRNA levels, which was prevented by 11-KT replacement. By contrast, LHβ mRNA levels were not changed by castration or 11-KT replacement. In addition, administration of 11-KT to sham-operated males suppressed the steady-state FSHβ and LHβ mRNA levels. These results indicate that 11-KT may function as a negative feedback regulator of FSHβ gene expression, and may act through the testis to down-regulate LHβ mRNA levels in male red seabream during this period. |
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