| 原位杂交检测鸡肿瘤病 |
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| 引用本文： | 王东东,顾节清,李春梅,宋延华.原位杂交检测鸡肿瘤病[J].中国畜牧兽医,2010,37(3):167-170. |
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| 作者姓名： | 王东东 顾节清 李春梅 宋延华 |
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| 作者单位： | （广东温氏食品集团研究院， 新兴 527400） |
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| 摘 要： | 本研究旨在运用原位杂交检测方法检测鸡的肿瘤病。根据GenBank中登录的MDV、ALV-J和REV参考毒株序列,设计并合成3对引物,经PCR方法扩增出相应的片段,并进行了克隆测序。将测序正确的片段亚克隆至pSPT-18,经转录得到DIG标记的特异cRNA探针。将探针分别与3种肿瘤病阳性组织石蜡切片进行杂交,可看到阳性信号,特异性、敏感性较好。运用该方法对32份疑似肿瘤病料进行了检测,MDV、ALV-J和REV的阳性率分别为25.00%、18.75%和3.13%。结果提示,鸡肿瘤病在鸡场中仍广泛存在。
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| 关 键 词： | 原位杂交 cRNA探针 地高辛 马立克氏病 禽白血病 禽网状内皮组织增殖病 |
Detection of Chicken Neoplastic Diseases by in Situ Hybridization |
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| WANG Dong-dong,GU Jie-qing,LI Chun-mei,SONG Yan-hua.Detection of Chicken Neoplastic Diseases by in Situ Hybridization[J].China Animal Husbandry & Veterinary Medicine,2010,37(3):167-170. |
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| Authors: | WANG Dong-dong GU Jie-qing LI Chun-mei SONG Yan-hua |
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| Institution: | (Guangdong Wen’s Food Group Academy, Xinxing 527400, China) |
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| Abstract: | This experiment was conducted to using a method of in situ hybridization to detect chicken neoplastic diseases.Three pairs of primers were designed and synthesized based on the published sequences of MDV,ALV-J and REV.The PCR amplified products were subcloned into pSPT-18 plasmid to generate DIG-labeled cRNA probes targeting MDV,ALV-J and REV respectively.Positive sections was hybridized with the three probes and the results showed good specificity and sensitivity.Thirty-two suspected neoplastic tissue samp... |
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| Keywords: | in situ hybridization cRNA probe DIG Marek's disease avain leukosis reticuloendotheliosis |
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