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| 鸡胚胎腺垂体促甲状腺激素细胞的发生及其变化 | |
| 引用本文： | 何玉琴,刘英,崔胜.鸡胚胎腺垂体促甲状腺激素细胞的发生及其变化[J].甘肃农业大学学报,2004,39(3):266-269. |
| 作者姓名： | 何玉琴 刘英 崔胜 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业大学生物学院,北京,100094 2. 甘肃农业大学动物医学院,甘肃,兰州,730070 3. 中国农业大学生物学院,北京,100094 |
| 基金项目： | 国家自然科学基金资助项目(编号:30170693，30325034) |
| 摘 要： | 应用免疫组织化学方法,对第3.5～20.5 d鸡胚腺垂体促甲状腺激素亚单位(TSH撸┫赴姆⑸⑹亢托翁植急浠媛山辛搜芯俊=峁允荆杭ε叻⒂牡?0.5 d,有少量TSH呦赴植加谙俅固迩耙叮赴派常伲舜螅患ε叻⒂牡?4.5 d以后,TSH呦赴灾黾樱≒ < 0.05),细胞体积增大,胞浆增多,细胞着色浓,分布于腺垂体前叶边缘区,少量分布于后叶背侧。研究结果证明,鸡胚腺垂体TSH呦赴⑸谂咛シ⒂闹衅冢幌赴脑鲋撤只谭⑸诩ε叻⒂闹泻笃冢籘SH的合成和分泌功能在胚胎发育的后期最旺盛。TSH呦赴植加谙俅固迩耙逗秃笠侗巢唷? |
| 关 键 词： | 鸡胚 腺垂体 促甲状腺激素亚单位(TSHβ)细胞 免疫组织化学 |
| 文章编号： | 1003-4315(2004)03-0266-04 |
Ontogeny and change of pituitary thyrotrophs of the chick embryonic |
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| HE Yu-qin,LIU Ying,CUI Sheng.Ontogeny and change of pituitary thyrotrophs of the chick embryonic[J].Journal of Gansu Agricultural University,2004,39(3):266-269. | |
| Authors: | HE Yu-qin LIU Ying CUI Sheng |
| Institution: | HE Yu-qin1,2,LIU Ying1,CUI Sheng2 |
| Abstract: | In order to determine the ontogeny and change of the chick embryonic pituitary thyrotrophs (TSH cells) during developing embryonic, the pituitary glands were collected at day 3.5 to 20.5 of incubation, respectively. The expression and distribution of pituitary TSH cells were then detected by immunohistochemical method. The experimental results demonstrated that few immunopositive TSH?cells were first detected in cephalic lobe of pituitary at 10.5 days, and then TSH cells numbers increased during the embryonic period sand widely distributed the fringe of cephalic lobe, and few TSH cells distributed dorsal of caudal. In the early stage, the volume of TSH cells was smaller, there was less cytoplasmic and bigger nuclear; and light staining. in the mid-incubation and later, the volume became bigger, the cells became intensive staining. These results suggest that ontogeny of TSH cells is in the middle stage of developing embryos; the proliferation and differentiation of TSH cells occur during middle late stage, the function of TSH cells is the most activity in the later stage. |
| Keywords: | chick embryo pituitary gland thyrotrophs (TSH cells) immunohistochemical |
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